DEVELOPMENT OF 14 MICROSATELLITE MARKERS IN ODONTITES VERNUS SL (OROBANCHACEAE) AND CROSS-AMPLIFICATION IN RELATED TAXA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890980" target="_blank" >RIV/60076658:12310/16:43890980 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.bioone.org/doi/10.3732/apps.1500111" target="_blank" >http://www.bioone.org/doi/10.3732/apps.1500111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3732/apps.1500111" target="_blank" >10.3732/apps.1500111</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DEVELOPMENT OF 14 MICROSATELLITE MARKERS IN ODONTITES VERNUS SL (OROBANCHACEAE) AND CROSS-AMPLIFICATION IN RELATED TAXA

Popis výsledku v původním jazyce

Premise of the study: Microsatellite primers were developed for the first time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene flow between different ploidy levels. Methods and Results: Fourteen polymorphic and reproducible loci were identified and optimized from O. vernus using a microsatellite-enriched library and 454 Junior sequencing. The set of primers amplified di- to pentanucleotide repeats and showed two to 13 alleles per locus. Transferability was tested in 30 taxa (19 belonging to Odontites and 11 from eight other genera of Orobanchaceae tribe Rhinantheae). Conclusions: The results indicate the utility of the newly developed microsatellites in O. vernus and several other species, which will be useful for taxon delimitation and conservation genetics studies.

Název v anglickém jazyce

DEVELOPMENT OF 14 MICROSATELLITE MARKERS IN ODONTITES VERNUS SL (OROBANCHACEAE) AND CROSS-AMPLIFICATION IN RELATED TAXA

Popis výsledku anglicky

Premise of the study: Microsatellite primers were developed for the first time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene flow between different ploidy levels. Methods and Results: Fourteen polymorphic and reproducible loci were identified and optimized from O. vernus using a microsatellite-enriched library and 454 Junior sequencing. The set of primers amplified di- to pentanucleotide repeats and showed two to 13 alleles per locus. Transferability was tested in 30 taxa (19 belonging to Odontites and 11 from eight other genera of Orobanchaceae tribe Rhinantheae). Conclusions: The results indicate the utility of the newly developed microsatellites in O. vernus and several other species, which will be useful for taxon delimitation and conservation genetics studies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP505%2F12%2F1390" target="_blank" >GAP505/12/1390: Biologie poloparazitů ze skupiny Rhinanthoidních Orobanchaceae: fyziologických procesů po ekologii společenstev</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applications in Plant Sciences

ISSN

2168-0450

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000378626400005

EID výsledku v databázi Scopus

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