The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895735" target="_blank" >RIV/60076658:12310/17:43895735 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/17:00480474

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elsc.201700014/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elsc.201700014/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elsc.201700014" target="_blank" >10.1002/elsc.201700014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light

Popis výsledku v původním jazyce

The green microalga Dictyosphaerium chlorelloides was identified as promising microorganism for biotechnological production of exopolysaccharides (EPS). In stationary phase the culture suspension solidifies to thick gel, with very high viscosity and high content of EPS which may be interesting for many biotechnological applications. To develop cultivation protocol for maximum biomass/polysaccharide production, the optimum conditions for growth and polysaccharides production were determined in this study using the crossed gradient cultivation method. Temperature and irradiance requirements of Dictyosphaerium chlorelloides were evaluated by statistical analyses for growth rate/biomass, extracellular (EPS) and intracellular (IPS) polysaccharides contents in crossed gradients of temperature (4-45 degrees C) and irradiance (2-18W/m(2), 9.1-82.3 mu mol/(m(2) s)). The maximum relative growth rate was observed at temperatures around 19.2 degrees C and relatively low irradiances in range 2.6-11W/m(2) (11.9-50.3 mu mol/(m(2) s)). The maximumIPS production was observed at temperatures around 19.2 degrees C and irradiance around 11 W/m(2) (50.3 mu mol/(m(2) s)). The maximum production of EPS was observed at temperatures around 25.7 degrees C and similar irradiances as IPS production. Due to temperature separation of growth and EPS production, development of cultivation protocol based controlled temperature manipulation is possible.

Název v anglickém jazyce

The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light

Popis výsledku anglicky

The green microalga Dictyosphaerium chlorelloides was identified as promising microorganism for biotechnological production of exopolysaccharides (EPS). In stationary phase the culture suspension solidifies to thick gel, with very high viscosity and high content of EPS which may be interesting for many biotechnological applications. To develop cultivation protocol for maximum biomass/polysaccharide production, the optimum conditions for growth and polysaccharides production were determined in this study using the crossed gradient cultivation method. Temperature and irradiance requirements of Dictyosphaerium chlorelloides were evaluated by statistical analyses for growth rate/biomass, extracellular (EPS) and intracellular (IPS) polysaccharides contents in crossed gradients of temperature (4-45 degrees C) and irradiance (2-18W/m(2), 9.1-82.3 mu mol/(m(2) s)). The maximum relative growth rate was observed at temperatures around 19.2 degrees C and relatively low irradiances in range 2.6-11W/m(2) (11.9-50.3 mu mol/(m(2) s)). The maximumIPS production was observed at temperatures around 19.2 degrees C and irradiance around 11 W/m(2) (50.3 mu mol/(m(2) s)). The maximum production of EPS was observed at temperatures around 25.7 degrees C and similar irradiances as IPS production. Due to temperature separation of growth and EPS production, development of cultivation protocol based controlled temperature manipulation is possible.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20900 - Industrial biotechnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Engineering in Life Sciences

ISSN

1618-0240

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1030-1038

Kód UT WoS článku

000412291600008

EID výsledku v databázi Scopus

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