Molecular survey of Rickettsial organisms in ectoparasites from a dog shelter in Northern Mexico

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895877" target="_blank" >RIV/60076658:12310/17:43895877 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/17:00483976

Výsledek na webu

<a href="https://ac.els-cdn.com/S2405939017301442/1-s2.0-S2405939017301442-main.pdf?_tid=ee0ae94a-ff58-11e7-bdfc-00000aab0f6c&acdnat=1516614488_bfbedb24073c60623217321ce7603d88" target="_blank" >https://ac.els-cdn.com/S2405939017301442/1-s2.0-S2405939017301442-main.pdf?_tid=ee0ae94a-ff58-11e7-bdfc-00000aab0f6c&acdnat=1516614488_bfbedb24073c60623217321ce7603d88</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vprsr.2017.10.005" target="_blank" >10.1016/j.vprsr.2017.10.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular survey of Rickettsial organisms in ectoparasites from a dog shelter in Northern Mexico

Popis výsledku v původním jazyce

The objective of this study was to screen and identify rickettsial organisms in ectoparasites collected from dogs in a shelter in Gomez Palacio, Durango, Mexico. One hundred dogs were inspected for ectoparasites. All the dogs were parasitized with Rhipicephalus sanguineus ticks, three with Heterodoxus spiniger lice and one with Ctenocephalides felis fleas. DNA was extracted from the ectoparasites found on each dog, and PCR with the primers for the Anaplasmataceae 16S rRNA and citrate synthase gltA genes were performed. Eight DNA samples obtained from ticks, three from lice and one from fleas were positive to 16S rRNA. Only one sample from C. felis and one from H. spiniger were positive to gltA. Sequence analysis of amplified products from C. felis showed identity to Rickettsia felis, Wolbachia pipientis, and Wolbachia spp., while a sequence from H. spiniger showed identity to Wolbachia spp. Herein we report the molecular detection of R. felis, W. pipientis, and Wolbachia spp. in C. felis and H. spiniger in northern Mexico. These results contribute to the knowledge of the microorganisms present in ectoparasites from dogs in Mexico.

Název v anglickém jazyce

Molecular survey of Rickettsial organisms in ectoparasites from a dog shelter in Northern Mexico

Popis výsledku anglicky

The objective of this study was to screen and identify rickettsial organisms in ectoparasites collected from dogs in a shelter in Gomez Palacio, Durango, Mexico. One hundred dogs were inspected for ectoparasites. All the dogs were parasitized with Rhipicephalus sanguineus ticks, three with Heterodoxus spiniger lice and one with Ctenocephalides felis fleas. DNA was extracted from the ectoparasites found on each dog, and PCR with the primers for the Anaplasmataceae 16S rRNA and citrate synthase gltA genes were performed. Eight DNA samples obtained from ticks, three from lice and one from fleas were positive to 16S rRNA. Only one sample from C. felis and one from H. spiniger were positive to gltA. Sequence analysis of amplified products from C. felis showed identity to Rickettsia felis, Wolbachia pipientis, and Wolbachia spp., while a sequence from H. spiniger showed identity to Wolbachia spp. Herein we report the molecular detection of R. felis, W. pipientis, and Wolbachia spp. in C. felis and H. spiniger in northern Mexico. These results contribute to the knowledge of the microorganisms present in ectoparasites from dogs in Mexico.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology: Regional Studies and Reports

ISSN

2405-9390

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

December 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

143-148

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85033553146