Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricomutum

Kód výsledku v IS VaVaI

RIV/60076658:12310/18:43897246 - isvavai.cz

Nalezeny alternativní kódy

RIV/60077344:_____/18:00495159

Výsledek na webu

https://reader.elsevier.com/reader/sd/AE632B7C93732B33EF877DB6EDEDA8049E50983AA4EAFC09D0C94461E8DA11AE6895F575B95B6BB4B1E8D6F0B7235544

DOI - Digital Object Identifier

10.1016/j.bbabio.2018.02.011

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricomutum

Popis výsledku v původním jazyce

We have applied femtosecond transient absorption spectroscopy in pump-probe and pump-dump-probe regimes to study energy transfer between fucoxanthin and Chl a in fucoxanthin-Chl a complex from the pennate diatom Phaeodactylum tricornutum. Experiments were carried out at room temperature and 77 K to reveal temperature dependence of energy transfer. At both temperatures, the ultrafast (< 100 fs) energy transfer channel from the fucoxanthin S-2 state is active and is complemented by the second pathway via the combined S-1/ICT state. The S-1/ICT-Chl a pathway has two channels, the fast one characterized by sub-picosecond energy transfer, and slow having time constants of 4.5 ps at room temperature and 6.6 ps at 77 K. The overall energy transfer via the S-1/ICT is faster at 77 K, because the fast component gains amplitude upon lowering the temperature. The pump dump-probe regime, with the dump pulse centered in the spectral region of ICT stimulated emission at 950 nm and applied at 2 ps after excitation, proved that the S-1 and ICT states of fucoxanthin in FCP are individual, yet coupled entities. Analysis of the pump-dump-probe data suggested that the main energy donor in the slow S-1/ICT-Chl a route is the S-1 part of the S-1/ICT potential surface.

Název v anglickém jazyce

Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricomutum

Popis výsledku anglicky

We have applied femtosecond transient absorption spectroscopy in pump-probe and pump-dump-probe regimes to study energy transfer between fucoxanthin and Chl a in fucoxanthin-Chl a complex from the pennate diatom Phaeodactylum tricornutum. Experiments were carried out at room temperature and 77 K to reveal temperature dependence of energy transfer. At both temperatures, the ultrafast (< 100 fs) energy transfer channel from the fucoxanthin S-2 state is active and is complemented by the second pathway via the combined S-1/ICT state. The S-1/ICT-Chl a pathway has two channels, the fast one characterized by sub-picosecond energy transfer, and slow having time constants of 4.5 ps at room temperature and 6.6 ps at 77 K. The overall energy transfer via the S-1/ICT is faster at 77 K, because the fast component gains amplitude upon lowering the temperature. The pump dump-probe regime, with the dump pulse centered in the spectral region of ICT stimulated emission at 950 nm and applied at 2 ps after excitation, proved that the S-1 and ICT states of fucoxanthin in FCP are individual, yet coupled entities. Analysis of the pump-dump-probe data suggested that the main energy donor in the slow S-1/ICT-Chl a route is the S-1 part of the S-1/ICT potential surface.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
S - Specificky vyzkum na vysokych skolach
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1859

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

357-365

Kód UT WoS článku

000430881200006

EID výsledku v databázi Scopus

2-s2.0-85043391297