Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897407" target="_blank" >RIV/60076658:12310/18:43897407 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00489356 RIV/00216224:14310/18:00100913

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0022191018300787?token=DE6D13E92A357D73805EF2EC4279838625701F67266BF7AB661E1494D2CA6C9A3A26DCC33E890E364012B34B3346FC90" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0022191018300787?token=DE6D13E92A357D73805EF2EC4279838625701F67266BF7AB661E1494D2CA6C9A3A26DCC33E890E364012B34B3346FC90</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jinsphys.2018.04.002" target="_blank" >10.1016/j.jinsphys.2018.04.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster

Popis výsledku v původním jazyce

This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh(1), AdoR(1) (adenosine receptor), and Akh(1) AdoR(1). Mortality decreased in all mutants post-EPN infection compared with the control (w(1118)). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w(1118) and Akh(1) larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR(1) and Akh(1) AdoR(1) mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh(1). In uninfected larvae mutations decreased antioxidative capacity in Akh(1) and increased in AdoR(1), however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w(1118) larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.

Název v anglickém jazyce

Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster

Popis výsledku anglicky

This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh(1), AdoR(1) (adenosine receptor), and Akh(1) AdoR(1). Mortality decreased in all mutants post-EPN infection compared with the control (w(1118)). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w(1118) and Akh(1) larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR(1) and Akh(1) AdoR(1) mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh(1). In uninfected larvae mutations decreased antioxidative capacity in Akh(1) and increased in AdoR(1), however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w(1118) larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA17-03253S" target="_blank" >GA17-03253S: Hormonální kontrola hmyzího obranného systému</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Insect Physiology

ISSN

0022-1910

e-ISSN

—

Svazek periodika

107

Číslo periodika v rámci svazku

MAY-JUN 2018

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

167-174

Kód UT WoS článku

000434751100021

EID výsledku v databázi Scopus

2-s2.0-85045386083