Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897417" target="_blank" >RIV/60076658:12310/18:43897417 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/18:00488384 RIV/00216224:14740/18:00100847

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201712284" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201712284</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/anie.201712284" target="_blank" >10.1002/anie.201712284</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Popis výsledku v původním jazyce

C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.

Název v anglickém jazyce

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Popis výsledku anglicky

C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF15_003%2F0000441" target="_blank" >EF15_003/0000441: Mechanismy a dynamika makromolekulárních komplexů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Angewandte Chemie International Edition

ISSN

1433-7851

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

2165-2169

Kód UT WoS článku

000424879400024

EID výsledku v databázi Scopus

2-s2.0-85041122273