Peripheral venous vs. capillary microfilariaemia in a dog co-infected with Dirofilaria repens and D-immitis: A comparative approach using triatomine bugs for blood collection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897512" target="_blank" >RIV/60076658:12310/18:43897512 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498789 RIV/62157124:16170/18:43876293 RIV/62157124:16810/18:43876293

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0304401718302103?token=D78C1D8D365187EA44F566FE5009BA20EE83C8BD1CB49EC1E28BBCC54DF71B8C5024F7E469E1B7C0EB833C01FA46255F" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0304401718302103?token=D78C1D8D365187EA44F566FE5009BA20EE83C8BD1CB49EC1E28BBCC54DF71B8C5024F7E469E1B7C0EB833C01FA46255F</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2018.05.017" target="_blank" >10.1016/j.vetpar.2018.05.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Peripheral venous vs. capillary microfilariaemia in a dog co-infected with Dirofilaria repens and D-immitis: A comparative approach using triatomine bugs for blood collection

Popis výsledku v původním jazyce

Dirofilaria immitis and D. repens are mosquito-borne nematodes, primarily infecting dogs, but also other species of carnivores and even humans. Given their impact on animal and human health, the transmission of these filarioids has been widely studied. The microfilariaemia has been shown to have a circadian variation for both Dirofilaria species infecting dogs. Due to methodological difficulties, the periodicity was only studied using venous blood samples, while the mosquitoes feed, in fact, on capillary blood. In this context, the present study aimed to test the feasibility of using triatomine bugs for the collection of capillary blood and to comparatively evaluate the level of microfilariaemia and its circadian variation in capillary blood vs. peripheral venous blood in a dog naturally co-infected with D. immitis and D. repens. The results showed a feeding success of 50%, with variations in the blood meal volume that the bugs ingested. The relative values of microfilariaemia (mf/bug) were strongly correlated with the volume of blood recovered: the more blood recovered from each bug, the higher values of microfilariaemia in the evening samples while the opposite results were obtained for the morning samples. The counting of microfilariae revealed a dominance of D. immitis in all the samples, but with significantly higher microfilariaemia in the venous blood. Meanwhile, for D. repens, the situation was opposite, with higher counts in the capillary blood samples. Our study showed that triatomine bugs can be used as a model for the collection and study of microfilariaemia in the capillary blood in mammals.s

Název v anglickém jazyce

Peripheral venous vs. capillary microfilariaemia in a dog co-infected with Dirofilaria repens and D-immitis: A comparative approach using triatomine bugs for blood collection

Popis výsledku anglicky

Dirofilaria immitis and D. repens are mosquito-borne nematodes, primarily infecting dogs, but also other species of carnivores and even humans. Given their impact on animal and human health, the transmission of these filarioids has been widely studied. The microfilariaemia has been shown to have a circadian variation for both Dirofilaria species infecting dogs. Due to methodological difficulties, the periodicity was only studied using venous blood samples, while the mosquitoes feed, in fact, on capillary blood. In this context, the present study aimed to test the feasibility of using triatomine bugs for the collection of capillary blood and to comparatively evaluate the level of microfilariaemia and its circadian variation in capillary blood vs. peripheral venous blood in a dog naturally co-infected with D. immitis and D. repens. The results showed a feeding success of 50%, with variations in the blood meal volume that the bugs ingested. The relative values of microfilariaemia (mf/bug) were strongly correlated with the volume of blood recovered: the more blood recovered from each bug, the higher values of microfilariaemia in the evening samples while the opposite results were obtained for the morning samples. The counting of microfilariae revealed a dominance of D. immitis in all the samples, but with significantly higher microfilariaemia in the venous blood. Meanwhile, for D. repens, the situation was opposite, with higher counts in the capillary blood samples. Our study showed that triatomine bugs can be used as a model for the collection and study of microfilariaemia in the capillary blood in mammals.s

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

—

Svazek periodika

257

Číslo periodika v rámci svazku

JUN 15 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

54-57

Kód UT WoS článku

000437816400010

EID výsledku v databázi Scopus

2-s2.0-85048009656