Insect fat body cell morphology and response to cold stress is modulated by acclimation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897537" target="_blank" >RIV/60076658:12310/18:43897537 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00495783

Výsledek na webu

<a href="http://jeb.biologists.org/content/221/21/jeb189647" target="_blank" >http://jeb.biologists.org/content/221/21/jeb189647</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jeb.189647" target="_blank" >10.1242/jeb.189647</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insect fat body cell morphology and response to cold stress is modulated by acclimation

Popis výsledku v původním jazyce

Mechanistic understanding about the nature of cellular cryoinjury and mechanisms by which some animals survive freezing while others do not is currently lacking. Here, we exploited the broadly manipulable freeze tolerance of larval malt flies (Chymomyza costata) to uncover cell and tissue morphological changes associated with freeze mortality. Diapause induction, cold acclimation and dietary proline supplementation generate malt fly variants ranging from weakly to extremely freeze tolerant. Using confocal microscopy and immunostaining of the fat body, Malpighian tubules and anterior midgut, we described tissue and cytoskeletal (F-actin and alpha-tubulin) morphologies among these variants after exposure to various cold stresses (from chilling at -5 degrees C to extreme freezing at -196 degrees C), and upon recovery from cold exposure. Fat body tissue appeared to be the most susceptible to cryoinjury: freezing caused coalescence of lipid droplets, loss of a-tubulin structure and apparent aggregation of F-actin. A combination of diapause and cold acclimation substantially lowered the temperature at which these morphological disruptions occurred. Larvae that recovered from a freezing challenge repaired F-actin aggregation but not lipid droplet coalescence or alpha-tubulin structure. Our observations indicate that lipid coalescence and damage to alpha-tubulin are non-lethal forms of freeze injury, and suggest that repair or removal (rather than protection) of actin proteins is a potential mechanism of acquired freeze tolerance.

Název v anglickém jazyce

Insect fat body cell morphology and response to cold stress is modulated by acclimation

Popis výsledku anglicky

Mechanistic understanding about the nature of cellular cryoinjury and mechanisms by which some animals survive freezing while others do not is currently lacking. Here, we exploited the broadly manipulable freeze tolerance of larval malt flies (Chymomyza costata) to uncover cell and tissue morphological changes associated with freeze mortality. Diapause induction, cold acclimation and dietary proline supplementation generate malt fly variants ranging from weakly to extremely freeze tolerant. Using confocal microscopy and immunostaining of the fat body, Malpighian tubules and anterior midgut, we described tissue and cytoskeletal (F-actin and alpha-tubulin) morphologies among these variants after exposure to various cold stresses (from chilling at -5 degrees C to extreme freezing at -196 degrees C), and upon recovery from cold exposure. Fat body tissue appeared to be the most susceptible to cryoinjury: freezing caused coalescence of lipid droplets, loss of a-tubulin structure and apparent aggregation of F-actin. A combination of diapause and cold acclimation substantially lowered the temperature at which these morphological disruptions occurred. Larvae that recovered from a freezing challenge repaired F-actin aggregation but not lipid droplet coalescence or alpha-tubulin structure. Our observations indicate that lipid coalescence and damage to alpha-tubulin are non-lethal forms of freeze injury, and suggest that repair or removal (rather than protection) of actin proteins is a potential mechanism of acquired freeze tolerance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA16-06374S" target="_blank" >GA16-06374S: Poškození hmyzího organismu nízkou teplotou a jeho náprava.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Biology

ISSN

0022-0949

e-ISSN

—

Svazek periodika

221

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000449824800024

EID výsledku v databázi Scopus

2-s2.0-85055855042