Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival Akt pathway

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897549" target="_blank" >RIV/60076658:12310/18:43897549 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498748

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0042682217303793?token=AE7DE1777BAE40C8829BF93C926D8F02E363602F4B798930CBC4C1C292D2CA27DE83E145D290B2E05EC80B1EA08D6CEF" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0042682217303793?token=AE7DE1777BAE40C8829BF93C926D8F02E363602F4B798930CBC4C1C292D2CA27DE83E145D290B2E05EC80B1EA08D6CEF</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.virol.2017.11.008" target="_blank" >10.1016/j.virol.2017.11.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival Akt pathway

Popis výsledku v původním jazyce

It has been suggested that tick saliva facilitates transmission of tick-borne encephalitis virus (TBEV) to vertebrates. The mechanism of this facilitation has not been elucidated yet. Since dendritic cells (DCs) are among first cells attacked by the virus, we examined the amount of virus and changes induced by saliva in TBEV-infected DCs. We found that virus replication was significantly increased by saliva of Ixodes ricinus tick. Next, saliva induced enhancement of Akt pathway activation was observed in TBEV-infected DCs. Akt mediated pathway is known for its anti-apoptotic and pro-survival effects. Accordingly, apoptosis of TBEV-infected DCs was declined and cellular viability increased in the presence of tick saliva. Saliva-induced enhancement of STAT1 and NF-kappa B was also observed in TBEV-infected DCs. In conclusion, we suggest that tick saliva provides pro-survival and anti-apoptotic signals to infected DCs via upregulation of Akt, which may have positive consequences for TBEV replication and transmission.

Název v anglickém jazyce

Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival Akt pathway

Popis výsledku anglicky

It has been suggested that tick saliva facilitates transmission of tick-borne encephalitis virus (TBEV) to vertebrates. The mechanism of this facilitation has not been elucidated yet. Since dendritic cells (DCs) are among first cells attacked by the virus, we examined the amount of virus and changes induced by saliva in TBEV-infected DCs. We found that virus replication was significantly increased by saliva of Ixodes ricinus tick. Next, saliva induced enhancement of Akt pathway activation was observed in TBEV-infected DCs. Akt mediated pathway is known for its anti-apoptotic and pro-survival effects. Accordingly, apoptosis of TBEV-infected DCs was declined and cellular viability increased in the presence of tick saliva. Saliva-induced enhancement of STAT1 and NF-kappa B was also observed in TBEV-infected DCs. In conclusion, we suggest that tick saliva provides pro-survival and anti-apoptotic signals to infected DCs via upregulation of Akt, which may have positive consequences for TBEV replication and transmission.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virology

ISSN

0042-6822

e-ISSN

—

Svazek periodika

514

Číslo periodika v rámci svazku

JAN 15 2018

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

98-105

Kód UT WoS článku

000423010200011

EID výsledku v databázi Scopus

2-s2.0-85034652583