Cyanobacteria inhabiting biological soil crusts of a polar desert: Sor Rondane Mountains, Antarctica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897562" target="_blank" >RIV/60076658:12310/18:43897562 - isvavai.cz</a>

Výsledek na webu

<a href="https://ac.els-cdn.com/S0723202018300274/1-s2.0-S0723202018300274-main.pdf?_tid=41ef0684-b473-4181-9d2a-aac4eda31658&acdnat=1546277006_cb83909028e3b210bb66b2aa9c61de70" target="_blank" >https://ac.els-cdn.com/S0723202018300274/1-s2.0-S0723202018300274-main.pdf?_tid=41ef0684-b473-4181-9d2a-aac4eda31658&acdnat=1546277006_cb83909028e3b210bb66b2aa9c61de70</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.syapm.2018.01.006" target="_blank" >10.1016/j.syapm.2018.01.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cyanobacteria inhabiting biological soil crusts of a polar desert: Sor Rondane Mountains, Antarctica

Popis výsledku v původním jazyce

Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sor Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs) that were put in place in 2010 at the time of sample collection and will be compared with BSC samples taken from the OTCs in the future. Cyanobacterial cell biovolume was estimated using epifluorescence microscopy, which revealed the dominance of filamentous cyanobacteria in all studied sites except the Utsteinen ridge, where unicellular cyanobacteria were the most abundant. Cyanobacterial diversity was studied by a combination of molecular fingerprinting methods based on the 16S rRNA gene (denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing) using cyanobacteria-specific primers. The number of DGGE sequences obtained per site was variable and, therefore, a high-throughput method was subsequently employed to improve the diversity coverage. Consistent with previous surveys in Antarctica, both methods showed that filamentous cyanobacteria, such as Leptolyngbya sp., Phormidium sp. and Microcoleus sp., were dominant in the studied sites. In addition, the studied localities differed in substrate type, climatic conditions and soil parameters, which probably resulted in differences in cyanobacterial community composition. Furthermore, the BSC growing on gneiss pebbles had lower cyanobacterial abundances than BSCs associated with granitic substrates. (C) 2018 Elsevier GmbH. All rights reserved.

Název v anglickém jazyce

Cyanobacteria inhabiting biological soil crusts of a polar desert: Sor Rondane Mountains, Antarctica

Popis výsledku anglicky

Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sor Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs) that were put in place in 2010 at the time of sample collection and will be compared with BSC samples taken from the OTCs in the future. Cyanobacterial cell biovolume was estimated using epifluorescence microscopy, which revealed the dominance of filamentous cyanobacteria in all studied sites except the Utsteinen ridge, where unicellular cyanobacteria were the most abundant. Cyanobacterial diversity was studied by a combination of molecular fingerprinting methods based on the 16S rRNA gene (denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing) using cyanobacteria-specific primers. The number of DGGE sequences obtained per site was variable and, therefore, a high-throughput method was subsequently employed to improve the diversity coverage. Consistent with previous surveys in Antarctica, both methods showed that filamentous cyanobacteria, such as Leptolyngbya sp., Phormidium sp. and Microcoleus sp., were dominant in the studied sites. In addition, the studied localities differed in substrate type, climatic conditions and soil parameters, which probably resulted in differences in cyanobacterial community composition. Furthermore, the BSC growing on gneiss pebbles had lower cyanobacterial abundances than BSCs associated with granitic substrates. (C) 2018 Elsevier GmbH. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/LM2010009" target="_blank" >LM2010009: Projekt CzechPolar - České polární stanice: Stavba a operační náklady</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Systematic and Applied Microbiology

ISSN

0723-2020

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

363-373

Kód UT WoS článku

000439923300012

EID výsledku v databázi Scopus

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