Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897587" target="_blank" >RIV/60076658:12310/18:43897587 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498839 RIV/61388971:_____/18:00490079

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S1434461017301062?token=19918AD415736D2BF6F3BFBB7E141AB6D4F002A09BCE6CD4DBA47FAAA2E749C944FFCBAA11FBB8B69A5EE05237068F8D" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S1434461017301062?token=19918AD415736D2BF6F3BFBB7E141AB6D4F002A09BCE6CD4DBA47FAAA2E749C944FFCBAA11FBB8B69A5EE05237068F8D</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.protis.2017.12.001" target="_blank" >10.1016/j.protis.2017.12.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia

Popis výsledku v původním jazyce

Chromera velia is an alveolate alga which represents the closest known phototrophic relative to apicomplexan parasites. Although the nuclear, mitochondrial, and plastid genomes of this alga have been sequenced, the number of chromosomes and ploidy of C. velia are unknown. We explored ploidy in the vegetative cell, the predominant stage in cultures of Chromera, using the tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH) in isolated nuclei of C. velia. Probes were derived from three single copy genes coding for 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME) kinase, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) synthase and Topoisomerase II. Our results indicate that the vegetative cell of C. velia is haploid, as each probe produced a single fluorescent signal, although the possibility of diploidy with somatic pairing of homologous chromosomes cannot be completely excluded. Restriction analysis and hybridization with the telomere probe produced eight bands suggesting the presence of four chromosomes in haploid vegetative cells of C. velia. However, when the chromerid-specific telomere probe (TTTAGGG)(4) was used for TSA-FISH, we consistently obtained a double signal. This may indicate that the four chromosomes are organized in clusters in interphase nuclei of C. velia, which is a chromosome organization similar to that of their apicomplexan relatives. (C) 2017 Elsevier GmbH. All rights reserved.

Název v anglickém jazyce

Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia

Popis výsledku anglicky

Chromera velia is an alveolate alga which represents the closest known phototrophic relative to apicomplexan parasites. Although the nuclear, mitochondrial, and plastid genomes of this alga have been sequenced, the number of chromosomes and ploidy of C. velia are unknown. We explored ploidy in the vegetative cell, the predominant stage in cultures of Chromera, using the tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH) in isolated nuclei of C. velia. Probes were derived from three single copy genes coding for 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME) kinase, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) synthase and Topoisomerase II. Our results indicate that the vegetative cell of C. velia is haploid, as each probe produced a single fluorescent signal, although the possibility of diploidy with somatic pairing of homologous chromosomes cannot be completely excluded. Restriction analysis and hybridization with the telomere probe produced eight bands suggesting the presence of four chromosomes in haploid vegetative cells of C. velia. However, when the chromerid-specific telomere probe (TTTAGGG)(4) was used for TSA-FISH, we consistently obtained a double signal. This may indicate that the four chromosomes are organized in clusters in interphase nuclei of C. velia, which is a chromosome organization similar to that of their apicomplexan relatives. (C) 2017 Elsevier GmbH. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protist

ISSN

1434-4610

e-ISSN

—

Svazek periodika

169

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

53-63

Kód UT WoS článku

000427418800005

EID výsledku v databázi Scopus

2-s2.0-85041563662