TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897643" target="_blank" >RIV/60076658:12310/18:43897643 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498156

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0166685118301622?token=504ADA88B60549E3A7BBD1ADCD9FE2E5B883203BD13B93F6870D5EE1C7727085C42368DA4325F8C054ACF7619324E90F" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0166685118301622?token=504ADA88B60549E3A7BBD1ADCD9FE2E5B883203BD13B93F6870D5EE1C7727085C42368DA4325F8C054ACF7619324E90F</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2018.09.003" target="_blank" >10.1016/j.molbiopara.2018.09.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei

Popis výsledku v původním jazyce

Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.

Název v anglickém jazyce

TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei

Popis výsledku anglicky

Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

—

Svazek periodika

225

Číslo periodika v rámci svazku

OCT 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

84-93

Kód UT WoS článku

000450378900011

EID výsledku v databázi Scopus

2-s2.0-85054167722