Cytotype variation, cryptic diversity and hybridization in Ranunculus sect. Batrachium revealed by flow cytometry and chromosome numbers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897707" target="_blank" >RIV/60076658:12310/18:43897707 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/18:00493235 RIV/00216208:11310/18:10380154

Výsledek na webu

<a href="http://www.preslia.cz/P183Prancl.pdf" target="_blank" >http://www.preslia.cz/P183Prancl.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.23855/preslia.2018.195" target="_blank" >10.23855/preslia.2018.195</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytotype variation, cryptic diversity and hybridization in Ranunculus sect. Batrachium revealed by flow cytometry and chromosome numbers

Popis výsledku v původním jazyce

Water-crowfoots (Ranunculus sect. Batrachium) are one of the taxonomically most intricate groups of aquatic plants. Investigation of its species is complicated by morphological reduction and phenotypic plasticity, as well as by the frequent occurrence of polyploidy and hybridization, giving rise to numerous intergrading or morphologically undetectable taxa. We used, for the first time in this group, flow cytometry to gain an insight into evolutionary processes and genome size differentiation in Ranunculus sect. Batrachium. Flow cytometry complemented by confirmation of chromosome counts was applied to an extensive dataset of 3354 individuals from 612 localities in 13 mainly central-European countries, of which the genome size was estimated for 1032 individuals. In total, 34 Batrachium cytotypes of five ploidy levels ranging from diploids to hexaploids were detected. The results indicate that the genome size is a reliable marker for distinguishing most of the traditionally recognized species, including those with identical chromosome numbers. Although variation in chromosome numbers is reported for six of eight central-European species, we detected only two taxa variable at the ploidy level (R. fluitans 2x + 3x, R. penicillatus 4x + 6x). However, the situation is much more challenging due to the presence of numerous hybrids and cryptic lineages. Cryptic variation was revealed within R. trichophyllus, in which three distinct tetraploid cytotypes were detected, which differ in their ecology and distribution. The allopolyploid complex of running-water taxa referred to as R. penicillatus is even more complex, including six different cytotypes. We also revealed 16 cytotypes of hybrid origin, which accounted for ca 15% of the individuals studied. Seven of the hybrid cytotypes were identified as F-1 hybrids and the rest are of unknown or uncertain origin. In the R. penicillatus group, the occurrence of a large hybrid swarm was documented. Hybrids with variable genome sizes frequently arise also from hybridization of the tetraploids R. peltatus and R. trichophyllus in which the gene flow tends to be largely unidirectional towards R. peltatus.

Název v anglickém jazyce

Cytotype variation, cryptic diversity and hybridization in Ranunculus sect. Batrachium revealed by flow cytometry and chromosome numbers

Popis výsledku anglicky

Water-crowfoots (Ranunculus sect. Batrachium) are one of the taxonomically most intricate groups of aquatic plants. Investigation of its species is complicated by morphological reduction and phenotypic plasticity, as well as by the frequent occurrence of polyploidy and hybridization, giving rise to numerous intergrading or morphologically undetectable taxa. We used, for the first time in this group, flow cytometry to gain an insight into evolutionary processes and genome size differentiation in Ranunculus sect. Batrachium. Flow cytometry complemented by confirmation of chromosome counts was applied to an extensive dataset of 3354 individuals from 612 localities in 13 mainly central-European countries, of which the genome size was estimated for 1032 individuals. In total, 34 Batrachium cytotypes of five ploidy levels ranging from diploids to hexaploids were detected. The results indicate that the genome size is a reliable marker for distinguishing most of the traditionally recognized species, including those with identical chromosome numbers. Although variation in chromosome numbers is reported for six of eight central-European species, we detected only two taxa variable at the ploidy level (R. fluitans 2x + 3x, R. penicillatus 4x + 6x). However, the situation is much more challenging due to the presence of numerous hybrids and cryptic lineages. Cryptic variation was revealed within R. trichophyllus, in which three distinct tetraploid cytotypes were detected, which differ in their ecology and distribution. The allopolyploid complex of running-water taxa referred to as R. penicillatus is even more complex, including six different cytotypes. We also revealed 16 cytotypes of hybrid origin, which accounted for ca 15% of the individuals studied. Seven of the hybrid cytotypes were identified as F-1 hybrids and the rest are of unknown or uncertain origin. In the R. penicillatus group, the occurrence of a large hybrid swarm was documented. Hybrids with variable genome sizes frequently arise also from hybridization of the tetraploids R. peltatus and R. trichophyllus in which the gene flow tends to be largely unidirectional towards R. peltatus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA17-06825S" target="_blank" >GA17-06825S: Kryptická variabilita vodních rostlin způsobená mezidruhovou hybridizací a polyploidizací</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Preslia : časopis České botanické společnosti

ISSN

0032-7786

e-ISSN

—

Svazek periodika

90

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

29

Strana od-do

195-223

Kód UT WoS článku

000442628500002

EID výsledku v databázi Scopus

2-s2.0-85052613892