The benign helminth Hymenolepis diminuta ameliorates chemically induced colitis in a rat model system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897715" target="_blank" >RIV/60076658:12310/18:43897715 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498711

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/parasitology/article/benign-helminth-hymenolepis-diminuta-ameliorates-chemically-induced-colitis-in-a-rat-model-system/508E75BE94E58E67251B216FECC06923" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/benign-helminth-hymenolepis-diminuta-ameliorates-chemically-induced-colitis-in-a-rat-model-system/508E75BE94E58E67251B216FECC06923</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182018000896" target="_blank" >10.1017/S0031182018000896</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The benign helminth Hymenolepis diminuta ameliorates chemically induced colitis in a rat model system

Popis výsledku v původním jazyce

The tapeworm Hymenolepis diminuta is a model for the impact of helminth colonization on the mammalian immune system and a candidate therapeutic agent for immune mediated inflammatory diseases (IMIDs). In mice, H. diminuta protects against models of inflammatory colitis by inducing a strong type 2 immune response that is activated to expel the immature worm. Rats are the definitive host of H. diminuta, and are colonized stably and over long time periods without harming the host. Rats mount a mild type 2 immune response to H. diminuta colonization, but this response does not generally ameliorate colitis. Here we investigate the ability of different life cycle stages of H. diminuta to protect rats against a model of colitis induced through application of the haptenizing agent dinitrobenzene sulphonic acid (DNBS) directly to the colon, and monitor rat clinical health, systemic inflammation measured by TNF alpha and IL-1 beta, and the gut microbiota. We show that immature H. diminuta induces a type 2 response as measured by increased IL-4, IL-13 and IL-10 expression, but does not protect against colitis. In contrast, rats colonized with mature H. diminuta and challenged with severe colitis (two applications of DNBS) have lower inflammation and less severe clinical symptoms. This effect is not related the initial type 2 immune response. The gut microbiota is disrupted during colitis and does not appear to play an overt role in H. diminuta-mediated protection.

Název v anglickém jazyce

The benign helminth Hymenolepis diminuta ameliorates chemically induced colitis in a rat model system

Popis výsledku anglicky

The tapeworm Hymenolepis diminuta is a model for the impact of helminth colonization on the mammalian immune system and a candidate therapeutic agent for immune mediated inflammatory diseases (IMIDs). In mice, H. diminuta protects against models of inflammatory colitis by inducing a strong type 2 immune response that is activated to expel the immature worm. Rats are the definitive host of H. diminuta, and are colonized stably and over long time periods without harming the host. Rats mount a mild type 2 immune response to H. diminuta colonization, but this response does not generally ameliorate colitis. Here we investigate the ability of different life cycle stages of H. diminuta to protect rats against a model of colitis induced through application of the haptenizing agent dinitrobenzene sulphonic acid (DNBS) directly to the colon, and monitor rat clinical health, systemic inflammation measured by TNF alpha and IL-1 beta, and the gut microbiota. We show that immature H. diminuta induces a type 2 response as measured by increased IL-4, IL-13 and IL-10 expression, but does not protect against colitis. In contrast, rats colonized with mature H. diminuta and challenged with severe colitis (two applications of DNBS) have lower inflammation and less severe clinical symptoms. This effect is not related the initial type 2 immune response. The gut microbiota is disrupted during colitis and does not appear to play an overt role in H. diminuta-mediated protection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

—

Svazek periodika

145

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1324-1335

Kód UT WoS článku

000443420600009

EID výsledku v databázi Scopus

2-s2.0-85048768156