Evaluating rodent experimental models for studies of Blastocystis ST1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897727" target="_blank" >RIV/60076658:12310/18:43897727 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498801

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0014489418301784?token=FF0A5AC063C89195C2A28D105497F005E9B6CBD81FA0772FA7D7FF6AC79E7789BE9A7AAAA6814E32715D0E91B5A1C6A0" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0014489418301784?token=FF0A5AC063C89195C2A28D105497F005E9B6CBD81FA0772FA7D7FF6AC79E7789BE9A7AAAA6814E32715D0E91B5A1C6A0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.exppara.2018.06.009" target="_blank" >10.1016/j.exppara.2018.06.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluating rodent experimental models for studies of Blastocystis ST1

Popis výsledku v původním jazyce

Blastocystis is a common inhabitant of the human gut, colonizing at least one billion people at a prevalence ranging from &lt; 10% to 100% in healthy human populations globally. The majority of carriers remain asymptomatic, suggesting that Blastocystis is largely a commensal, though Blastocystis has also been implicated in disease in some people. However, there are no in vivo model systems in which to experimentally test the impact of Blastocystis on mammalian hosts and the gut ecosystem and determine which factors underlie these variable clinical outcomes. We evaluated a rat model for sustaining of a human-derived Blastocystis ST1 and assess colonization success and longevity. Because of the broad host range of Blastocystis, we compared the rat with three other rodent species to establish the reproducibility of our method. Blastocystis was introduced by esophageal gavage and colonization success evaluated by Blastocystis culture. Culture was also used to determine that all animals were negative prior to colonization and negative controls remain Blastocystis-free. In this study, Blastocystis ST1 established in 100% of the outbred rats (Rattus norvegicus) and gerbils (Meriones unguiculatus) challenged. Rats were colonized asymptomatically for more than one year, but Blastocystis ST1 was not transmitted between rats. Mus musculus strain CD1 and Mastomys coucha were not susceptible to Blastocystis ST1. Thus, rats appear to be a suitable in vivo model for studies of Blastocystis ST1, as do gerbils though testing was less extensive. This work lays the foundation for experimental work on the role of Blastocystis in health and disease.

Název v anglickém jazyce

Evaluating rodent experimental models for studies of Blastocystis ST1

Popis výsledku anglicky

Blastocystis is a common inhabitant of the human gut, colonizing at least one billion people at a prevalence ranging from &lt; 10% to 100% in healthy human populations globally. The majority of carriers remain asymptomatic, suggesting that Blastocystis is largely a commensal, though Blastocystis has also been implicated in disease in some people. However, there are no in vivo model systems in which to experimentally test the impact of Blastocystis on mammalian hosts and the gut ecosystem and determine which factors underlie these variable clinical outcomes. We evaluated a rat model for sustaining of a human-derived Blastocystis ST1 and assess colonization success and longevity. Because of the broad host range of Blastocystis, we compared the rat with three other rodent species to establish the reproducibility of our method. Blastocystis was introduced by esophageal gavage and colonization success evaluated by Blastocystis culture. Culture was also used to determine that all animals were negative prior to colonization and negative controls remain Blastocystis-free. In this study, Blastocystis ST1 established in 100% of the outbred rats (Rattus norvegicus) and gerbils (Meriones unguiculatus) challenged. Rats were colonized asymptomatically for more than one year, but Blastocystis ST1 was not transmitted between rats. Mus musculus strain CD1 and Mastomys coucha were not susceptible to Blastocystis ST1. Thus, rats appear to be a suitable in vivo model for studies of Blastocystis ST1, as do gerbils though testing was less extensive. This work lays the foundation for experimental work on the role of Blastocystis in health and disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Parasitology

ISSN

0014-4894

e-ISSN

—

Svazek periodika

191

Číslo periodika v rámci svazku

AUG 2018

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

55-61

Kód UT WoS článku

000441369900008

EID výsledku v databázi Scopus

2-s2.0-85049931024