Heterotrophic N-2-fixation contributes to nitrogen economy of a common wetland sedge, Schoenoplectus californicus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43898441" target="_blank" >RIV/60076658:12310/18:43898441 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00495233

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0195570" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0195570</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0195570" target="_blank" >10.1371/journal.pone.0195570</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heterotrophic N-2-fixation contributes to nitrogen economy of a common wetland sedge, Schoenoplectus californicus

Popis výsledku v původním jazyce

A survey of the ecological variability within 52 populations of Schoenoplectus californicus (C.A. Mey.) Sojak across its distributional range revealed that it is commonly found in nitrogen (N) limited areas, but rarely in phosphorus limited soils. We explored the hypothesis that S. californicus supplements its nitrogen demand by bacterial N-2-fixation processes associated with its roots and rhizomes. We estimated N-2-fixation of diazotrophs associated with plant rhizomes and roots from several locations throughout the species&apos; range and conducted an experiment growing plants in zero, low, and high N additions. Nitrogenase activity in rhizomes and roots was measured using the acetylene reduction assay. The presence of diazotrophs was verified by the detection of the nifHgene. Nitrogenase activity was restricted to rhizomes and roots and it was two orders of magnitude higher in the latter plant organs (81 and 2032 nmol C2H4 g DW-1 d(-1), respectively). Correspondingly, 40x more nifH gene copies were found on roots compared to rhizomes. The proportion of the nifHgene copies in total bacterial DNA was positively correlated with the nitrogenase activity. In the experiment, the contribution of fixed N to the plant N content ranged from 13.8% to 32.5% among clones from different locations. These are relatively high values for a non-cultivated plant and justify future research on the link between N-fixing bacteria and S. californicus production.

Název v anglickém jazyce

Heterotrophic N-2-fixation contributes to nitrogen economy of a common wetland sedge, Schoenoplectus californicus

Popis výsledku anglicky

A survey of the ecological variability within 52 populations of Schoenoplectus californicus (C.A. Mey.) Sojak across its distributional range revealed that it is commonly found in nitrogen (N) limited areas, but rarely in phosphorus limited soils. We explored the hypothesis that S. californicus supplements its nitrogen demand by bacterial N-2-fixation processes associated with its roots and rhizomes. We estimated N-2-fixation of diazotrophs associated with plant rhizomes and roots from several locations throughout the species&apos; range and conducted an experiment growing plants in zero, low, and high N additions. Nitrogenase activity in rhizomes and roots was measured using the acetylene reduction assay. The presence of diazotrophs was verified by the detection of the nifHgene. Nitrogenase activity was restricted to rhizomes and roots and it was two orders of magnitude higher in the latter plant organs (81 and 2032 nmol C2H4 g DW-1 d(-1), respectively). Correspondingly, 40x more nifH gene copies were found on roots compared to rhizomes. The proportion of the nifHgene copies in total bacterial DNA was positively correlated with the nitrogenase activity. In the experiment, the contribution of fixed N to the plant N content ranged from 13.8% to 32.5% among clones from different locations. These are relatively high values for a non-cultivated plant and justify future research on the link between N-fixing bacteria and S. californicus production.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

22

Strana od-do

—

Kód UT WoS článku

000430660600016

EID výsledku v databázi Scopus

2-s2.0-85045952060