Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899487" target="_blank" >RIV/60076658:12310/19:43899487 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/19:00507526 RIV/61389030:_____/19:00507526 RIV/61989100:27710/19:10242266

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007%2Fs11120-019-00628-7.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs11120-019-00628-7.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11120-019-00628-7" target="_blank" >10.1007/s11120-019-00628-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Popis výsledku v původním jazyce

We suggest a new technique for estimating the relative drawdown of CO2 concentration (c) in the intercellular air space (IAS) across hypostomatous leaves (expressed as the ratio c(d)/c(b), where the indexes d and b denote the adaxial and abaxial edges, respectively, of IAS), based on the carbon isotope composition (delta C-13) of leaf cuticular membranes (CMs), cuticular waxes (WXs) or epicuticular waxes (EWXs) isolated from opposite leaf sides. The relative drawdown in the intracellular liquid phase (i.e., the ratio c(c)/c(bd), where c(c) and c(bd) stand for mean CO2 concentrations in chloroplasts and in the IAS), the fraction of intercellular resistance in the total mesophyll resistance (r(IAS)/r(m)), leaf thickness, and leaf mass per area (LMA) were also assessed. We show in a conceptual model that the upper (adaxial) side of a hypostomatous leaf should be enriched in C-13 compared to the lower (abaxial) side. CM, WX, and/or EWX isolated from 40 hypostomatous C-3 species were C-13 depleted relative to bulk leaf tissue by 2.01-2.85 parts per thousand. The difference in delta C-13 between the abaxial and adaxial leaf sides (delta C-13(AB)-C-13(AD), Delta(b-d)), ranged from -2.22 to +0.71 parts per thousand (-0.09 +/- 0.54 parts per thousand, mean +/- SD) in CM and from -7.95 to 0.89 parts per thousand (-1.17 +/- 1.40 parts per thousand) in WX. In contrast, two tested amphistomatous species showed no significant Delta(b-d) difference in WX. Delta(b-d) correlated negatively with LMA and leaf thickness of hypostomatous leaves, which indicates that the mesophyll air space imposes a non-negligible resistance to CO2 diffusion. delta C-13 of EWX and 30-C aldehyde in WX reveal a stronger CO2 drawdown than bulk WX or CM. Mean values of c(d)/c(b) and c(c)/c(bd) were 0.90 +/- 0.12 and 0.66 +/- 0.11, respectively, across 14 investigated species in which wax was isolated and analyzed. The diffusion resistance of IAS contributed 20 +/- 14% to total mesophyll resistance and reflects species-specific and environmentally-induced differences in leaf functional anatomy.

Název v anglickém jazyce

Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Popis výsledku anglicky

We suggest a new technique for estimating the relative drawdown of CO2 concentration (c) in the intercellular air space (IAS) across hypostomatous leaves (expressed as the ratio c(d)/c(b), where the indexes d and b denote the adaxial and abaxial edges, respectively, of IAS), based on the carbon isotope composition (delta C-13) of leaf cuticular membranes (CMs), cuticular waxes (WXs) or epicuticular waxes (EWXs) isolated from opposite leaf sides. The relative drawdown in the intracellular liquid phase (i.e., the ratio c(c)/c(bd), where c(c) and c(bd) stand for mean CO2 concentrations in chloroplasts and in the IAS), the fraction of intercellular resistance in the total mesophyll resistance (r(IAS)/r(m)), leaf thickness, and leaf mass per area (LMA) were also assessed. We show in a conceptual model that the upper (adaxial) side of a hypostomatous leaf should be enriched in C-13 compared to the lower (abaxial) side. CM, WX, and/or EWX isolated from 40 hypostomatous C-3 species were C-13 depleted relative to bulk leaf tissue by 2.01-2.85 parts per thousand. The difference in delta C-13 between the abaxial and adaxial leaf sides (delta C-13(AB)-C-13(AD), Delta(b-d)), ranged from -2.22 to +0.71 parts per thousand (-0.09 +/- 0.54 parts per thousand, mean +/- SD) in CM and from -7.95 to 0.89 parts per thousand (-1.17 +/- 1.40 parts per thousand) in WX. In contrast, two tested amphistomatous species showed no significant Delta(b-d) difference in WX. Delta(b-d) correlated negatively with LMA and leaf thickness of hypostomatous leaves, which indicates that the mesophyll air space imposes a non-negligible resistance to CO2 diffusion. delta C-13 of EWX and 30-C aldehyde in WX reveal a stronger CO2 drawdown than bulk WX or CM. Mean values of c(d)/c(b) and c(c)/c(bd) were 0.90 +/- 0.12 and 0.66 +/- 0.11, respectively, across 14 investigated species in which wax was isolated and analyzed. The diffusion resistance of IAS contributed 20 +/- 14% to total mesophyll resistance and reflects species-specific and environmentally-induced differences in leaf functional anatomy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthesis Research

ISSN

0166-8595

e-ISSN

—

Svazek periodika

141

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

19

Strana od-do

33-51

Kód UT WoS článku

000474427900003

EID výsledku v databázi Scopus

2-s2.0-85062465091