Spectroscopic Properties of Violaxanthin and Lutein Triplet States in LHCII are Independent of Carotenoid Composition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899725" target="_blank" >RIV/60076658:12310/19:43899725 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/19:00518123

Výsledek na webu

<a href="https://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.9b06293" target="_blank" >https://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.9b06293</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.jpcb.9b06293" target="_blank" >10.1021/acs.jpcb.9b06293</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spectroscopic Properties of Violaxanthin and Lutein Triplet States in LHCII are Independent of Carotenoid Composition

Popis výsledku v původním jazyce

Chlorophyll triplet excited states are byproducts of photosynthetic processes that can indirectly harm biological membranes by forming highly reactive oxygen species. A crucial photoprotective mechanism evolved by plants to counter this threat involves the triplet energy transfer from chlorophylls to carotenoid molecules, in which triplet states are not reactive. In the major light-harvesting complex of photosystem II (LHCII), the two central luteins play an important role in the mechanism, but it has been shown that carotenoid triplets are formed even when other carotenoids replace them in their binding sites. In this work, we have investigated carotenoid triplet formation in LHCII isolated from Arabidopsis thaliana npq1lut2 plants, in which violaxanthin replaces lutein. Although transient absorption spectroscopy showed altered singlet excited-state dynamics in the mutant LHCII without lutein, these antennae formed carotenoid triplets that were spectrally and dynamically identical to the wild-type protein. We conclude that lutein-binding sites in LHCII have conserved characteristics to ensure efficient triplet energy transfer to the carotenoid molecules that they accommodate, making the identity of the carotenoid trivial per se.

Název v anglickém jazyce

Spectroscopic Properties of Violaxanthin and Lutein Triplet States in LHCII are Independent of Carotenoid Composition

Popis výsledku anglicky

Chlorophyll triplet excited states are byproducts of photosynthetic processes that can indirectly harm biological membranes by forming highly reactive oxygen species. A crucial photoprotective mechanism evolved by plants to counter this threat involves the triplet energy transfer from chlorophylls to carotenoid molecules, in which triplet states are not reactive. In the major light-harvesting complex of photosystem II (LHCII), the two central luteins play an important role in the mechanism, but it has been shown that carotenoid triplets are formed even when other carotenoids replace them in their binding sites. In this work, we have investigated carotenoid triplet formation in LHCII isolated from Arabidopsis thaliana npq1lut2 plants, in which violaxanthin replaces lutein. Although transient absorption spectroscopy showed altered singlet excited-state dynamics in the mutant LHCII without lutein, these antennae formed carotenoid triplets that were spectrally and dynamically identical to the wild-type protein. We conclude that lutein-binding sites in LHCII have conserved characteristics to ensure efficient triplet energy transfer to the carotenoid molecules that they accommodate, making the identity of the carotenoid trivial per se.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GX19-28323X" target="_blank" >GX19-28323X: Vztah mezi strukturou a funkcí karotenoidů: Nové cesty k řešení nezodpovězených otázek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physical Chemistry B

ISSN

1520-6106

e-ISSN

—

Svazek periodika

123

Číslo periodika v rámci svazku

44

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

9312-9320

Kód UT WoS článku

000495772000004

EID výsledku v databázi Scopus

2-s2.0-85074409774