Wwc2Is a Novel Cell Division Regulator During Preimplantation Mouse Embryo Lineage Formation and Oogenesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901140" target="_blank" >RIV/60076658:12310/20:43901140 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985904:_____/20:00533450
Výsledek na webu
<a href="https://pubmed.ncbi.nlm.nih.gov/33042987/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/33042987/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcell.2020.00857" target="_blank" >10.3389/fcell.2020.00857</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Wwc2Is a Novel Cell Division Regulator During Preimplantation Mouse Embryo Lineage Formation and Oogenesis
Popis výsledku v původním jazyce
Formation of the hatching mouse blastocyst marks the end of preimplantation development, whereby previous cell cleavages culminate in the formation of three distinct cell lineages (trophectoderm, primitive endoderm and epiblast). We report that dysregulated expression ofWwc2, a genetic paralog ofKibra/Wwc1(a known activator of Hippo-signaling, a key pathway during preimplantation development), is specifically associated with cell autonomous deficits in embryo cell number and cell division abnormalities. Division phenotypes are also observed during mouse oocyte meiotic maturation, asWwc2dysregulation blocks progression to the stage of meiosis II metaphase (MII) arrest and is associated with spindle defects and failed Aurora-A kinase (AURKA) activation. Oocyte and embryo cell division defects, each occurring in the absence of centrosomes, are fully reversible by expression of recombinant HA-epitope tagged WWC2, restoring activated oocyte AURKA levels. Additionally, clonal embryonic dysregulation implicatesWwc2in maintaining the pluripotent epiblast lineage. Thus,Wwc2is a novel regulator of meiotic and early mitotic cell divisions, and mouse blastocyst cell fate.
Název v anglickém jazyce
Wwc2Is a Novel Cell Division Regulator During Preimplantation Mouse Embryo Lineage Formation and Oogenesis
Popis výsledku anglicky
Formation of the hatching mouse blastocyst marks the end of preimplantation development, whereby previous cell cleavages culminate in the formation of three distinct cell lineages (trophectoderm, primitive endoderm and epiblast). We report that dysregulated expression ofWwc2, a genetic paralog ofKibra/Wwc1(a known activator of Hippo-signaling, a key pathway during preimplantation development), is specifically associated with cell autonomous deficits in embryo cell number and cell division abnormalities. Division phenotypes are also observed during mouse oocyte meiotic maturation, asWwc2dysregulation blocks progression to the stage of meiosis II metaphase (MII) arrest and is associated with spindle defects and failed Aurora-A kinase (AURKA) activation. Oocyte and embryo cell division defects, each occurring in the absence of centrosomes, are fully reversible by expression of recombinant HA-epitope tagged WWC2, restoring activated oocyte AURKA levels. Additionally, clonal embryonic dysregulation implicatesWwc2in maintaining the pluripotent epiblast lineage. Thus,Wwc2is a novel regulator of meiotic and early mitotic cell divisions, and mouse blastocyst cell fate.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-02891S" target="_blank" >GA18-02891S: Regulaci rovnováhy mezi diferenciace a pluripotenci; charakterizace funkce p38-MAPK ve vnitřní buněčné hmotě myší blastocysty na molekulární úrovni.</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Frontiers in Cell and Developmental Biology
ISSN
2296-634X
e-ISSN
—
Svazek periodika
8
Číslo periodika v rámci svazku
SEP 17 2020
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
20
Strana od-do
—
Kód UT WoS článku
000576814000001
EID výsledku v databázi Scopus
2-s2.0-85091940514