Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901183" target="_blank" >RIV/60076658:12310/20:43901183 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/20:00537498 RIV/00027162:_____/20:N0000105

Výsledek na webu

<a href="https://www.mdpi.com/1999-4915/12/8/902" target="_blank" >https://www.mdpi.com/1999-4915/12/8/902</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v12080902" target="_blank" >10.3390/v12080902</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

Popis výsledku v původním jazyce

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) andIxodes ricinustick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

Název v anglickém jazyce

Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

Popis výsledku anglicky

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) andIxodes ricinustick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses - Basel

ISSN

1999-4915

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

—

Kód UT WoS článku

000579648500001

EID výsledku v databázi Scopus

2-s2.0-85089798271