Dual Substrate Specificity of the Rutinosidase from Aspergillus nigerand the Role of Its Substrate Tunnel

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901219" target="_blank" >RIV/60076658:12310/20:43901219 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00532691 RIV/61388963:_____/20:00532691

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/21/16/5671" target="_blank" >https://www.mdpi.com/1422-0067/21/16/5671</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21165671" target="_blank" >10.3390/ijms21165671</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dual Substrate Specificity of the Rutinosidase from Aspergillus nigerand the Role of Its Substrate Tunnel

Popis výsledku v původním jazyce

Rutinosidases (alpha-l-rhamnopyranosyl-(1-6)-beta-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone. Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation). The recombinant rutinosidase fromAspergillus niger(AnRut) is selectively produced inPichia pastoris.It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation. This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits beta-d-glucopyranosidase activity. As a result, new compounds are formed by beta-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3). Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields. Fourteen compounds were synthesized by glucosylation or rutinosylation of selected acceptors. The products were isolated and structurally characterized. Interactions between the transglycosylation products and the recombinantAnRut were analyzed by molecular modeling. We revealed the role of a substrate tunnel in the structure ofAnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).

Název v anglickém jazyce

Dual Substrate Specificity of the Rutinosidase from Aspergillus nigerand the Role of Its Substrate Tunnel

Popis výsledku anglicky

Rutinosidases (alpha-l-rhamnopyranosyl-(1-6)-beta-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone. Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation). The recombinant rutinosidase fromAspergillus niger(AnRut) is selectively produced inPichia pastoris.It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation. This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits beta-d-glucopyranosidase activity. As a result, new compounds are formed by beta-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3). Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields. Fourteen compounds were synthesized by glucosylation or rutinosylation of selected acceptors. The products were isolated and structurally characterized. Interactions between the transglycosylation products and the recombinantAnRut were analyzed by molecular modeling. We revealed the role of a substrate tunnel in the structure ofAnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

—

Kód UT WoS článku

000565060500001

EID výsledku v databázi Scopus

—