Ontogeny, species identity, and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901259" target="_blank" >RIV/60076658:12310/20:43901259 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/20:00533610

Výsledek na webu

<a href="https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-020-00921-x" target="_blank" >https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-020-00921-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40168-020-00921-x" target="_blank" >10.1186/s40168-020-00921-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ontogeny, species identity, and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae)

Popis výsledku v původním jazyce

Background Kissing bugs (Triatominae) are blood-feeding insects best known as the vectors ofTrypanosoma cruzi, the causative agent of Chagas&apos; disease. Considering the high epidemiological relevance of these vectors, their biology and bacterial symbiosis remains surprisingly understudied. While previous investigations revealed generally low individual complexity but high among-individual variability of the triatomine microbiomes, any consistent microbiome determinants have not yet been identified across multiple Triatominae species. Methods To obtain a more comprehensive view of triatomine microbiomes, we investigated the host-microbiome relationship of fiveTriatomaspecies sampled from white-throated woodrat (Neotoma albigula) nests in multiple locations across the USA. We applied optimised 16S rRNA gene metabarcoding with a novel 18S rRNA gene blocking primer to a set of 170T.cruzi-negative individuals across all six instars. Results Triatomine gut microbiome composition is strongly influenced by three principal factors: ontogeny, species identity, and the environment. The microbiomes are characterised by significant loss in bacterial diversity throughout ontogenetic development. First instars possess the highest bacterial diversity while adult microbiomes are routinely dominated by a single taxon. Primarily, the bacterial genusDietziadominates late-stage nymphs and adults ofT.rubida,T.protracta, andT.lecticulariabut is not present in the phylogenetically more distantT.gerstaeckeriandT.sanguisuga. Species-specific microbiome composition, particularly pronounced in early instars, is further modulated by locality-specific effects. In addition, pathogenic bacteria of the genusBartonella, acquired from the vertebrate hosts, are an abundant component ofTriatomamicrobiomes. Conclusion Our study is the first to demonstrate deterministic patterns in microbiome composition among all life stages and multipleTriatomaspecies. We hypothesise that triatomine microbiome assemblages are produced by species- and life stage-dependent uptake of environmental bacteria and multiple indirect transmission strategies that promote bacterial transfer between individuals. Altogether, our study highlights the complexity of Triatominae symbiosis with bacteria and warrant further investigation to understand microbiome function in these important vectors.

Název v anglickém jazyce

Ontogeny, species identity, and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae)

Popis výsledku anglicky

Background Kissing bugs (Triatominae) are blood-feeding insects best known as the vectors ofTrypanosoma cruzi, the causative agent of Chagas&apos; disease. Considering the high epidemiological relevance of these vectors, their biology and bacterial symbiosis remains surprisingly understudied. While previous investigations revealed generally low individual complexity but high among-individual variability of the triatomine microbiomes, any consistent microbiome determinants have not yet been identified across multiple Triatominae species. Methods To obtain a more comprehensive view of triatomine microbiomes, we investigated the host-microbiome relationship of fiveTriatomaspecies sampled from white-throated woodrat (Neotoma albigula) nests in multiple locations across the USA. We applied optimised 16S rRNA gene metabarcoding with a novel 18S rRNA gene blocking primer to a set of 170T.cruzi-negative individuals across all six instars. Results Triatomine gut microbiome composition is strongly influenced by three principal factors: ontogeny, species identity, and the environment. The microbiomes are characterised by significant loss in bacterial diversity throughout ontogenetic development. First instars possess the highest bacterial diversity while adult microbiomes are routinely dominated by a single taxon. Primarily, the bacterial genusDietziadominates late-stage nymphs and adults ofT.rubida,T.protracta, andT.lecticulariabut is not present in the phylogenetically more distantT.gerstaeckeriandT.sanguisuga. Species-specific microbiome composition, particularly pronounced in early instars, is further modulated by locality-specific effects. In addition, pathogenic bacteria of the genusBartonella, acquired from the vertebrate hosts, are an abundant component ofTriatomamicrobiomes. Conclusion Our study is the first to demonstrate deterministic patterns in microbiome composition among all life stages and multipleTriatomaspecies. We hypothesise that triatomine microbiome assemblages are produced by species- and life stage-dependent uptake of environmental bacteria and multiple indirect transmission strategies that promote bacterial transfer between individuals. Altogether, our study highlights the complexity of Triatominae symbiosis with bacteria and warrant further investigation to understand microbiome function in these important vectors.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GJ18-24707Y" target="_blank" >GJ18-24707Y: Význam a ekologická stabilita mikrobiomů krevsajících ploštic podčeledi Triatominae, přenašečů Chagasovy nemoci</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbiome

ISSN

2049-2618

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

—

Kód UT WoS článku

000576637200001

EID výsledku v databázi Scopus

2-s2.0-85092491628