A Study on the Prevalence and Subtype Diversity of the Intestinal Protist Blastocystissp. in a Gut-Healthy Human Population in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901284" target="_blank" >RIV/60076658:12310/20:43901284 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/20:00538647

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fcimb.2020.544335/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcimb.2020.544335/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcimb.2020.544335" target="_blank" >10.3389/fcimb.2020.544335</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Study on the Prevalence and Subtype Diversity of the Intestinal Protist Blastocystissp. in a Gut-Healthy Human Population in the Czech Republic

Popis výsledku v původním jazyce

Blastocystissp. is a common intestinal protist colonizing the human intestine the prevalence of which varies across non-industrialized and industrialized countries. Its role in the human gut ecosystem remains unclear due to persisting gaps in knowledge of epidemiology and factors affecting gut colonization. Here, we aimed to expand the knowledge of the epidemiology ofBlastocystissp. in the gut-healthy humans in one of the industrialized European countries, including the distribution of its subtypes, the correlation between its occurrence and several factors such as lifestyle, contact with animals, age, and sex. A total of 288 stool samples were obtained from asymptomatic individuals over the entire age-range and 136 samples from animals with which the volunteers were in frequent contact. All samples were examined in parallel by PCR and xenicin vitroculture.Blastocystissp. was detected in samples from both human and non-human hosts. In humans, the overall prevalence was 24% and eight subtypes were found; in animals, the prevalence was 10%, and only five subtypes were detected. A higher incidence ofBlastocystissp. was observed in individuals (i) traveling outside Europe, (ii) in frequent contact with livestock, and (iii) over 50 years of age. We found no effect on gender onBlastocystissp. colonization. This study provides data on the prevalence and diversity of the gut protistBlastocystissp. and its subtypes in a gut-healthy human population with emphasis on several factors such as contact with animals, lifestyle, age, and gender.

Název v anglickém jazyce

A Study on the Prevalence and Subtype Diversity of the Intestinal Protist Blastocystissp. in a Gut-Healthy Human Population in the Czech Republic

Popis výsledku anglicky

Blastocystissp. is a common intestinal protist colonizing the human intestine the prevalence of which varies across non-industrialized and industrialized countries. Its role in the human gut ecosystem remains unclear due to persisting gaps in knowledge of epidemiology and factors affecting gut colonization. Here, we aimed to expand the knowledge of the epidemiology ofBlastocystissp. in the gut-healthy humans in one of the industrialized European countries, including the distribution of its subtypes, the correlation between its occurrence and several factors such as lifestyle, contact with animals, age, and sex. A total of 288 stool samples were obtained from asymptomatic individuals over the entire age-range and 136 samples from animals with which the volunteers were in frequent contact. All samples were examined in parallel by PCR and xenicin vitroculture.Blastocystissp. was detected in samples from both human and non-human hosts. In humans, the overall prevalence was 24% and eight subtypes were found; in animals, the prevalence was 10%, and only five subtypes were detected. A higher incidence ofBlastocystissp. was observed in individuals (i) traveling outside Europe, (ii) in frequent contact with livestock, and (iii) over 50 years of age. We found no effect on gender onBlastocystissp. colonization. This study provides data on the prevalence and diversity of the gut protistBlastocystissp. and its subtypes in a gut-healthy human population with emphasis on several factors such as contact with animals, lifestyle, age, and gender.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/EF16_019%2F0000759" target="_blank" >EF16_019/0000759: Centrum výzkumu patogenity a virulence parazitů</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Cellular and Infection Microbiology

ISSN

2235-2988

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

OCT 6 2020

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

000579481300001

EID výsledku v databázi Scopus

2-s2.0-85093365124