Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901347" target="_blank" >RIV/60076658:12310/20:43901347 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/20:00531531

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acschembio.0c00178" target="_blank" >https://pubs.acs.org/doi/10.1021/acschembio.0c00178</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acschembio.0c00178" target="_blank" >10.1021/acschembio.0c00178</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation

Popis výsledku v původním jazyce

Dinucleoside polyphosphates (Np(n)Ns) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5&apos; RNA caps in Escherichia coll. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed Np(n)Ns to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of Ap(n)Gs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.

Název v anglickém jazyce

Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation

Popis výsledku anglicky

Dinucleoside polyphosphates (Np(n)Ns) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5&apos; RNA caps in Escherichia coll. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed Np(n)Ns to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of Ap(n)Gs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LL1603" target="_blank" >LL1603: Virální RNA modifikace - Esenciální kroky v chemické evoluci proteinových kofaktorů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Chemical Biology

ISSN

1554-8929

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1765-1772

Kód UT WoS článku

000551550000006

EID výsledku v databázi Scopus

2-s2.0-85088279012