Identification of Immune Regulatory Genes inApis melliferathrough Caffeine Treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901409" target="_blank" >RIV/60076658:12310/20:43901409 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/20:00532213

Výsledek na webu

<a href="https://www.mdpi.com/2075-4450/11/8/516" target="_blank" >https://www.mdpi.com/2075-4450/11/8/516</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/insects11080516" target="_blank" >10.3390/insects11080516</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of Immune Regulatory Genes inApis melliferathrough Caffeine Treatment

Popis výsledku v původním jazyce

Simple Summary The preference of honeybees to consume nectar with caffeine has been recorded. To investigate the effects of caffeine to this important pollinator, we first focus on the influences on immunity which is seldom explored in insects regarding caffeine. In our results, we discovered the suppressive effects on viral pathogens and the boosting effects on immunity after caffeine treatment. At least six different latent-infecting viruses in Taiwan were suppressed by caffeine. Nevertheless, the enhancement on immunity may not be effective if the bees have not been exposed to the environment or potential natural secondary metabolites like caffeine. These findings provide a basic but valuable insight into how caffeine can aid honeybees in fighting against viral invasion. Plants and pollinators are mutually beneficial: plants provide nectar as a food source and in return their pollen is disseminated by pollinators such as honeybees. Some plants secrete chemicals to deter herbivores as a protective measure, among which is caffeine, a naturally occurring, bitter tasting, and pharmacologically active secondary compound. It can be found in low concentrations in the nectars of some plants and as such, when pollinators consume nectar, they also take in small amounts of caffeine. Whilst caffeine has been indicated as an antioxidant in both mammals and insects, the effect on insect immunity is unclear. In the present study, honeybees were treated with caffeine and the expression profiles of genes involved in immune responses were measured to evaluate the influence of caffeine on immunity. In addition, honeybees were infected with deformed wing virus (DWV) to study how caffeine affects their response against pathogens. Our results showed that caffeine can increase the expression of genes involved in immunity and reduce virus copy numbers, indicating that it has the potential to help honeybees fight against viral infection. The present study provides a valuable insight into the mechanism by which honeybees react to biotic stress and how caffeine can serve as a positive contributor, thus having a potential application in beekeeping.

Název v anglickém jazyce

Identification of Immune Regulatory Genes inApis melliferathrough Caffeine Treatment

Popis výsledku anglicky

Simple Summary The preference of honeybees to consume nectar with caffeine has been recorded. To investigate the effects of caffeine to this important pollinator, we first focus on the influences on immunity which is seldom explored in insects regarding caffeine. In our results, we discovered the suppressive effects on viral pathogens and the boosting effects on immunity after caffeine treatment. At least six different latent-infecting viruses in Taiwan were suppressed by caffeine. Nevertheless, the enhancement on immunity may not be effective if the bees have not been exposed to the environment or potential natural secondary metabolites like caffeine. These findings provide a basic but valuable insight into how caffeine can aid honeybees in fighting against viral invasion. Plants and pollinators are mutually beneficial: plants provide nectar as a food source and in return their pollen is disseminated by pollinators such as honeybees. Some plants secrete chemicals to deter herbivores as a protective measure, among which is caffeine, a naturally occurring, bitter tasting, and pharmacologically active secondary compound. It can be found in low concentrations in the nectars of some plants and as such, when pollinators consume nectar, they also take in small amounts of caffeine. Whilst caffeine has been indicated as an antioxidant in both mammals and insects, the effect on insect immunity is unclear. In the present study, honeybees were treated with caffeine and the expression profiles of genes involved in immune responses were measured to evaluate the influence of caffeine on immunity. In addition, honeybees were infected with deformed wing virus (DWV) to study how caffeine affects their response against pathogens. Our results showed that caffeine can increase the expression of genes involved in immunity and reduce virus copy numbers, indicating that it has the potential to help honeybees fight against viral infection. The present study provides a valuable insight into the mechanism by which honeybees react to biotic stress and how caffeine can serve as a positive contributor, thus having a potential application in beekeeping.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insects

ISSN

2075-4450

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

000564170900001

EID výsledku v databázi Scopus

2-s2.0-85090658677