Substrate-dependent incorporation of carbon and hydrogen for lipid biosynthesis by Methanosarcina barkeri

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901447" target="_blank" >RIV/60076658:12310/20:43901447 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/20:00536304

Výsledek na webu

<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.12876" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.12876</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1758-2229.12876" target="_blank" >10.1111/1758-2229.12876</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Substrate-dependent incorporation of carbon and hydrogen for lipid biosynthesis by Methanosarcina barkeri

Popis výsledku v původním jazyce

Dual stable isotope probing has been used to infer rates of microbial biomass production and modes of carbon fixation. In order to validate this approach for assessing archaeal production, the methanogenic archaeonMethanosarcina barkeriwas grown either with H-2, acetate or methanol with D2O and(13)C-dissolved inorganic carbon (DIC). Our results revealed unexpectedly low D incorporation into lipids, with the net fraction of water-derived hydrogen amounting to 0.357 +/- 0.042, 0.226 +/- 0.003 and 0.393 +/- 0.029 for growth on H-2/CO2, acetate and methanol respectively. The variability in net water H assimilation into lipids during the growth ofM.barkerion different substrates is possibly attributed to different Gibbs free energy yields, such that higher energy yield promoted the exchange of hydrogen between medium water and lipids. Because NADPH likely serves as the portal for H transfer, increased NADPH production and/or turnover associated with high energy yield may explain the apparent differences in net water H assimilation into lipids. The variable DIC and water H incorporation intoM.barkerilipids imply systematic, metabolic patterns of isotope incorporation and suggest that the ratio of(13)C-DIC versus D2O assimilation in environmental samples may serve as a proxy for microbial energetics in addition to microbial production and carbon assimilation pathways.

Název v anglickém jazyce

Substrate-dependent incorporation of carbon and hydrogen for lipid biosynthesis by Methanosarcina barkeri

Popis výsledku anglicky

Dual stable isotope probing has been used to infer rates of microbial biomass production and modes of carbon fixation. In order to validate this approach for assessing archaeal production, the methanogenic archaeonMethanosarcina barkeriwas grown either with H-2, acetate or methanol with D2O and(13)C-dissolved inorganic carbon (DIC). Our results revealed unexpectedly low D incorporation into lipids, with the net fraction of water-derived hydrogen amounting to 0.357 +/- 0.042, 0.226 +/- 0.003 and 0.393 +/- 0.029 for growth on H-2/CO2, acetate and methanol respectively. The variability in net water H assimilation into lipids during the growth ofM.barkerion different substrates is possibly attributed to different Gibbs free energy yields, such that higher energy yield promoted the exchange of hydrogen between medium water and lipids. Because NADPH likely serves as the portal for H transfer, increased NADPH production and/or turnover associated with high energy yield may explain the apparent differences in net water H assimilation into lipids. The variable DIC and water H incorporation intoM.barkerilipids imply systematic, metabolic patterns of isotope incorporation and suggest that the ratio of(13)C-DIC versus D2O assimilation in environmental samples may serve as a proxy for microbial energetics in addition to microbial production and carbon assimilation pathways.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology Reports

ISSN

1758-2229

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

555-567

Kód UT WoS článku

000571477600010

EID výsledku v databázi Scopus

2-s2.0-85091199654