Energy transfer pathways in the CAC light-harvesting complex of Rhodomonas salina

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901844" target="_blank" >RIV/60076658:12310/20:43901844 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00536787

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0005272820301304?token=E4092301933B14D8ED51A28F5CBAA5C1F271EDD8F3E2529F61E3C573F687245A3A2BCE82055E8191CC5526EF8B646E00" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0005272820301304?token=E4092301933B14D8ED51A28F5CBAA5C1F271EDD8F3E2529F61E3C573F687245A3A2BCE82055E8191CC5526EF8B646E00</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2020.148280" target="_blank" >10.1016/j.bbabio.2020.148280</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Energy transfer pathways in the CAC light-harvesting complex of Rhodomonas salina

Popis výsledku v původním jazyce

Photosynthetic organisms had to evolve diverse mechanisms of light-harvesting to supply photosynthetic apparatus with enough energy. Cryptophytes represent one of the groups of photosynthetic organisms combining external and internal antenna systems. They contain one type of immobile phycobiliprotein located at the lumenal side of the thylakoid membrane, together with membrane-bound chlorophyll a/c antenna (CAC). Here we employ femtosecond transient absorption spectroscopy to study energy transfer pathways in the CAC proteins of cryptophyte Rhodomonas salina. The major CAC carotenoid, alloxanthin, is a cryptophyte-specific carotenoid, and it is the only naturally-occurring carotenoid with two triple bonds in its structure. In order to explore the energy transfer pathways within the CAC complex, three excitation wavelengths (505, 590, and 640 nm) were chosen to excite pigments in the CAC antenna. The excitation of Chl c at either 590 or 640 nm proves efficient energy transfer between Chl c and Chl a. The excitation of alloxanthin at 505 nm shows an active pathway from the S2 state with efficiency around 50%, feeding both Chl a and Chl c with approximately 1:1 branching ratio, yet, the S1-route is rather inefficient. The 57 ps energy transfer time to Chl a gives ~25% efficiency of the S1 channel. The low efficiency of the S1 route renders the overall carotenoid-Chl energy transfer efficiency low, pointing to the regulatory role of alloxanthin in the CAC antenna. © 2020 Elsevier B.V.

Název v anglickém jazyce

Energy transfer pathways in the CAC light-harvesting complex of Rhodomonas salina

Popis výsledku anglicky

Photosynthetic organisms had to evolve diverse mechanisms of light-harvesting to supply photosynthetic apparatus with enough energy. Cryptophytes represent one of the groups of photosynthetic organisms combining external and internal antenna systems. They contain one type of immobile phycobiliprotein located at the lumenal side of the thylakoid membrane, together with membrane-bound chlorophyll a/c antenna (CAC). Here we employ femtosecond transient absorption spectroscopy to study energy transfer pathways in the CAC proteins of cryptophyte Rhodomonas salina. The major CAC carotenoid, alloxanthin, is a cryptophyte-specific carotenoid, and it is the only naturally-occurring carotenoid with two triple bonds in its structure. In order to explore the energy transfer pathways within the CAC complex, three excitation wavelengths (505, 590, and 640 nm) were chosen to excite pigments in the CAC antenna. The excitation of Chl c at either 590 or 640 nm proves efficient energy transfer between Chl c and Chl a. The excitation of alloxanthin at 505 nm shows an active pathway from the S2 state with efficiency around 50%, feeding both Chl a and Chl c with approximately 1:1 branching ratio, yet, the S1-route is rather inefficient. The 57 ps energy transfer time to Chl a gives ~25% efficiency of the S1 channel. The low efficiency of the S1 route renders the overall carotenoid-Chl energy transfer efficiency low, pointing to the regulatory role of alloxanthin in the CAC antenna. © 2020 Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA19-11494S" target="_blank" >GA19-11494S: Role mikrodoménové organizace tylakoidní mebrány ve fotosyntéze</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1861

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

000562511300014

EID výsledku v databázi Scopus

2-s2.0-85089187774