Components of the G(s) signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta(2)-adrenergic receptor activation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43902459" target="_blank" >RIV/60076658:12310/20:43902459 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333016/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333016/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tra.12724" target="_blank" >10.1111/tra.12724</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Components of the G(s) signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta(2)-adrenergic receptor activation

Popis výsledku v původním jazyce

The G protein signaling cascade is a key player in cell signaling. Cascade activation leads to a redistribution of its members in various cellular compartments. These changes are likely related to the &quot;second wave&quot; of signaling from endosomes. Here, we set out to determine whether G(s) signaling cascade members expressed at very low levels exhibit altered mobility and localize in clathrin-coated structures (CCSs) or caveolae upon activation by beta(2)-adrenergic receptors (beta(2)AR). Activated beta(2)AR showed decreased mobility and sustained accumulation in CCSs but not in caveolae. Arrestin 3 translocated to the plasma membrane after beta(2)AR activation and showed very low mobility and pronounced accumulation in CCSs. In contrast, G alpha(s) and G gamma(2) exhibited a modest reduction in mobility but no detectable accumulation in or exclusion from CCSs or caveolae. The effector adenylyl cyclase 5 (AC5) showed a slight mobility increase upon beta(2)AR stimulation, no redistribution to CCSs, and weak activation-independent accumulation in caveolae. Our findings show an overall decrease in the mobility of most activated G(s) signaling cascade members and confirm that beta(2)AR and arrestin 3 accumulate in CCSs, while G alpha(s), G gamma(2) and AC5 can transiently enter CCSs and caveolae but do not accumulate in and are not excluded from these domains.

Název v anglickém jazyce

Components of the G(s) signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta(2)-adrenergic receptor activation

Popis výsledku anglicky

The G protein signaling cascade is a key player in cell signaling. Cascade activation leads to a redistribution of its members in various cellular compartments. These changes are likely related to the &quot;second wave&quot; of signaling from endosomes. Here, we set out to determine whether G(s) signaling cascade members expressed at very low levels exhibit altered mobility and localize in clathrin-coated structures (CCSs) or caveolae upon activation by beta(2)-adrenergic receptors (beta(2)AR). Activated beta(2)AR showed decreased mobility and sustained accumulation in CCSs but not in caveolae. Arrestin 3 translocated to the plasma membrane after beta(2)AR activation and showed very low mobility and pronounced accumulation in CCSs. In contrast, G alpha(s) and G gamma(2) exhibited a modest reduction in mobility but no detectable accumulation in or exclusion from CCSs or caveolae. The effector adenylyl cyclase 5 (AC5) showed a slight mobility increase upon beta(2)AR stimulation, no redistribution to CCSs, and weak activation-independent accumulation in caveolae. Our findings show an overall decrease in the mobility of most activated G(s) signaling cascade members and confirm that beta(2)AR and arrestin 3 accumulate in CCSs, while G alpha(s), G gamma(2) and AC5 can transiently enter CCSs and caveolae but do not accumulate in and are not excluded from these domains.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GJ20-11563Y" target="_blank" >GJ20-11563Y: Alosterická komunikace v membránových proteinových komplexech a její propojení s konformační dynamikou na mnoha časových škálách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Traffic

ISSN

1398-9219

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

324-332

Kód UT WoS článku

000518877400002

EID výsledku v databázi Scopus

2-s2.0-85081303588