Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903157" target="_blank" >RIV/60076658:12310/21:43903157 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/21:PU140688 RIV/60077344:_____/21:00553178 RIV/00216208:11110/21:10428050

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0924424721001059?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0924424721001059?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sna.2021.112643" target="_blank" >10.1016/j.sna.2021.112643</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Popis výsledku v původním jazyce

Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of approximate to 14 nW. The results showed that the defensive behavior by the ejection of the trichocysts&apos; content consumed energy of approximate to 0.75 mJ per cell, and the dissipated power was approximate to 303.8 mu W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Popis výsledku anglicky

Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of approximate to 14 nW. The results showed that the defensive behavior by the ejection of the trichocysts&apos; content consumed energy of approximate to 0.75 mJ per cell, and the dissipated power was approximate to 303.8 mu W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators A: Physical

ISSN

0924-4247

e-ISSN

—

Svazek periodika

323

Číslo periodika v rámci svazku

JUN 1 2021

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000635598600013

EID výsledku v databázi Scopus

2-s2.0-85102398216