A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903167" target="_blank" >RIV/60076658:12310/21:43903167 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00554452

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/15476286.2021.1940445?scroll=top&needAccess=true" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/15476286.2021.1940445?scroll=top&needAccess=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15476286.2021.1940445" target="_blank" >10.1080/15476286.2021.1940445</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

Popis výsledku v původním jazyce

In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA(Trp)) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA(Trp), this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of Trypanosoma brucei for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in T. brucei. The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.

Název v anglickém jazyce

A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

Popis výsledku anglicky

In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA(Trp)) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA(Trp), this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of Trypanosoma brucei for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in T. brucei. The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA Biology

ISSN

1547-6286

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

Suplement 1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

278-286

Kód UT WoS článku

000669722800001

EID výsledku v databázi Scopus

2-s2.0-85109619571