High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903224" target="_blank" >RIV/60076658:12310/21:43903224 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00118817 RIV/62156489:43210/21:43918392

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1470160X20309481?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1470160X20309481?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecolind.2020.107009" target="_blank" >10.1016/j.ecolind.2020.107009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Popis výsledku v původním jazyce

Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerske Hory Mountains (Czech Republic). In total, we detected 68-103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Cantina yidgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.

Název v anglickém jazyce

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Popis výsledku anglicky

Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerske Hory Mountains (Czech Republic). In total, we detected 68-103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Cantina yidgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10612 - Mycology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Ecological Indicators

ISSN

1470-160X

e-ISSN

—

Svazek periodika

121

Číslo periodika v rámci svazku

FEB 2021

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000604882200013

EID výsledku v databázi Scopus

2-s2.0-85092156379