In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903675" target="_blank" >RIV/60076658:12310/21:43903675 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652079:_____/21:00542600

Výsledek na webu

<a href="https://www.mdpi.com/2073-4395/11/3/596" target="_blank" >https://www.mdpi.com/2073-4395/11/3/596</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy11030596" target="_blank" >10.3390/agronomy11030596</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

Popis výsledku v původním jazyce

The aim of this work was to find an effective protocol for in vitro propagation and to perform the in vitro polyploidization of diploid Thymus vulgaris (2n = 30) using two experimental methods based on the use of oryzalin, an antimitotic agent. The ploidy level of the obtained shoots was checked by flow cytometric analysis. The most efficient conditions for inducing polyploidy were oryzalin concentrations of 0.346 and 1.73 mg L-1 present in the medium for two weeks. The vital polyploid shoots were multiplied for further evaluation, rooting and final transfer to nonsterile glasshouse and field conditions. The chemical compositions of the essential oils (EOs)-which were obtained from dried field grown plants by steam distillation-were analyzed by gas chromatography/mass spectrometry (GC/MS). The identified substances contributed approximately 95% to the total peak area. Statistical analysis revealed that the tetraploid subclone and the diploid reference plant do not differ in total terpene content, but they do differ in the relative proportions of all the individual terpenes with the exception of alpha-pinene and UN5, indicating that both clones produce EOs of different quality. The obtained results showed the possibility of developing more efficient botanical insecticides based on EOs obtained from the tetraploid plants.

Název v anglickém jazyce

In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

Popis výsledku anglicky

The aim of this work was to find an effective protocol for in vitro propagation and to perform the in vitro polyploidization of diploid Thymus vulgaris (2n = 30) using two experimental methods based on the use of oryzalin, an antimitotic agent. The ploidy level of the obtained shoots was checked by flow cytometric analysis. The most efficient conditions for inducing polyploidy were oryzalin concentrations of 0.346 and 1.73 mg L-1 present in the medium for two weeks. The vital polyploid shoots were multiplied for further evaluation, rooting and final transfer to nonsterile glasshouse and field conditions. The chemical compositions of the essential oils (EOs)-which were obtained from dried field grown plants by steam distillation-were analyzed by gas chromatography/mass spectrometry (GC/MS). The identified substances contributed approximately 95% to the total peak area. Statistical analysis revealed that the tetraploid subclone and the diploid reference plant do not differ in total terpene content, but they do differ in the relative proportions of all the individual terpenes with the exception of alpha-pinene and UN5, indicating that both clones produce EOs of different quality. The obtained results showed the possibility of developing more efficient botanical insecticides based on EOs obtained from the tetraploid plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 - Rozvoj Centra pro studium dopadů globální změny klimatu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agronomy

ISSN

2073-4395

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000633192200001

EID výsledku v databázi Scopus

2-s2.0-85108842407