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Regulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol-4-kinases beta 1 and beta 2 in Arabidopsis thaliana

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43906089" target="_blank" >RIV/60076658:12310/21:43906089 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/60461373:22330/21:43922233

  • Výsledek na webu

    <a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.202000223" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.202000223</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/pmic.202000223" target="_blank" >10.1002/pmic.202000223</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Regulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol-4-kinases beta 1 and beta 2 in Arabidopsis thaliana

  • Popis výsledku v původním jazyce

    Phosphatidylinositol-4-kinases beta 1 and beta 2 (PI4K beta 1/PI4K beta 2), which are responsible for phosphorylation of phosphatidylinositol to phosphatidylinositol-4-phosphate, have important roles in plant vesicular trafficking. Moreover, PI4K beta 1/PI4K beta 2 negatively regulates biosynthesis of phytohormone salicylic acid (SA), a key player in plant immune responses. The study focused on the effect of PI4K beta 1/PI4K beta 2 deficiency and SA level on the proteome of microsomal fraction. For that purpose we used four Arabidopsis thaliana genotypes: wild type; double mutant with impaired function of PI4K beta 1/PI4K beta 2 (pi4k beta 1/pi4k beta 2) exhibiting high SA level; sid2 mutant with impaired SA biosynthesis depending on the isochorismate synthase 1 and triple mutant sid2/pi4k beta 1/pi4k beta 2. We identified 1797 proteins whose levels were changed between genotypes. We showed that increased SA concentration affected the levels of 473 proteins. This includes typical SA pathway markers but also points to connections between SA pathway and clathrin-independent endocytosis (flotillins) and exocytosis/protein secretion (syntaxins, tetraspanin) to be investigated in future. In contrast to SA, the absence of PI4K beta 1/PI4K beta 2 itself affected only 27 proteins. Among them we identified CERK1, a receptor for chitin. Although PI4K beta 1/PI4K beta 2 deficiency itself did not have a substantial impact on the proteome of the microsomal fraction, our data clearly show that it enhances proteome changes when SA pathway is modulated in parallel.

  • Název v anglickém jazyce

    Regulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol-4-kinases beta 1 and beta 2 in Arabidopsis thaliana

  • Popis výsledku anglicky

    Phosphatidylinositol-4-kinases beta 1 and beta 2 (PI4K beta 1/PI4K beta 2), which are responsible for phosphorylation of phosphatidylinositol to phosphatidylinositol-4-phosphate, have important roles in plant vesicular trafficking. Moreover, PI4K beta 1/PI4K beta 2 negatively regulates biosynthesis of phytohormone salicylic acid (SA), a key player in plant immune responses. The study focused on the effect of PI4K beta 1/PI4K beta 2 deficiency and SA level on the proteome of microsomal fraction. For that purpose we used four Arabidopsis thaliana genotypes: wild type; double mutant with impaired function of PI4K beta 1/PI4K beta 2 (pi4k beta 1/pi4k beta 2) exhibiting high SA level; sid2 mutant with impaired SA biosynthesis depending on the isochorismate synthase 1 and triple mutant sid2/pi4k beta 1/pi4k beta 2. We identified 1797 proteins whose levels were changed between genotypes. We showed that increased SA concentration affected the levels of 473 proteins. This includes typical SA pathway markers but also points to connections between SA pathway and clathrin-independent endocytosis (flotillins) and exocytosis/protein secretion (syntaxins, tetraspanin) to be investigated in future. In contrast to SA, the absence of PI4K beta 1/PI4K beta 2 itself affected only 27 proteins. Among them we identified CERK1, a receptor for chitin. Although PI4K beta 1/PI4K beta 2 deficiency itself did not have a substantial impact on the proteome of the microsomal fraction, our data clearly show that it enhances proteome changes when SA pathway is modulated in parallel.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10608 - Biochemistry and molecular biology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Proteomics

  • ISSN

    1615-9853

  • e-ISSN

    1615-9861

  • Svazek periodika

    21

  • Číslo periodika v rámci svazku

    5

  • Stát vydavatele periodika

    US - Spojené státy americké

  • Počet stran výsledku

    6

  • Strana od-do

    nestrankovano

  • Kód UT WoS článku

    000620571400001

  • EID výsledku v databázi Scopus

    2-s2.0-85101281948