Use of environmental sequencing in evaluating fungal response to peatland degradation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F24%3A43908501" target="_blank" >RIV/60076658:12310/24:43908501 - isvavai.cz</a>

Výsledek na webu

<a href="http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/http://mires-and-peat.net/media/map31/map_31_17.pdf" target="_blank" >http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/http://mires-and-peat.net/media/map31/map_31_17.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.19189/MaP.2023.OMB.Sc.2319915" target="_blank" >10.19189/MaP.2023.OMB.Sc.2319915</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of environmental sequencing in evaluating fungal response to peatland degradation

Popis výsledku v původním jazyce

Central European peatlands are critically endangered by climate change, eutrophication, disturbed water regime and inappropriate management. This leads to their degradation, which affects all components of these habitats including fungi, and significantly fungi participating in the carbon cycle such as saprotrophs, mycorrhizal symbionts and parasites. Nature conservation efforts focus mainly on macrofungi, especially &apos;fungi important for nature conservation&apos; (FINC) which are rare or associated with endangered habitats. To find out whether standard environmental DNA (eDNA) sequencing methods can be used to monitor the effect of peatland degradation on FINC and fungal communities, we established pairs of plots in degraded and wellpreserved parts of ten peatlands in the T &amp; rcaron;ebo &amp; ncaron; Protected Landscape Area (Czech Republic). Peatland degradation was locally caused by a combination of different factors (water level and chemistry) followed by vegetation succession, whose effect on fungi was evaluated using multivariate analyses. A total of 28 FINC species were detected using macrofungal surveying, whereas 25 were found using eDNA of Sphagnum and peat (Illumina sequencing of ITS2). These two methods agreed on 13 predominantly abundant FINC species, most of which grew in both well-preserved and degraded plots. However, their sensitivity to the studied degradation could not be assessed due to the low abundance of most species. Fungal communities found on the basis of both methods (macrofungal survey, eDNA) were affected by water chemistry, whereas a significant effect of vegetation and locally expansive plants was demonstrated only in fungal communities found using macrofungal survey. Optimisation (e.g. specific primers) for local conditions leading to a better FINC capture must be carried out if eDNA is to be used in the practice of evaluating management effects on peatlands.

Název v anglickém jazyce

Use of environmental sequencing in evaluating fungal response to peatland degradation

Popis výsledku anglicky

Central European peatlands are critically endangered by climate change, eutrophication, disturbed water regime and inappropriate management. This leads to their degradation, which affects all components of these habitats including fungi, and significantly fungi participating in the carbon cycle such as saprotrophs, mycorrhizal symbionts and parasites. Nature conservation efforts focus mainly on macrofungi, especially &apos;fungi important for nature conservation&apos; (FINC) which are rare or associated with endangered habitats. To find out whether standard environmental DNA (eDNA) sequencing methods can be used to monitor the effect of peatland degradation on FINC and fungal communities, we established pairs of plots in degraded and wellpreserved parts of ten peatlands in the T &amp; rcaron;ebo &amp; ncaron; Protected Landscape Area (Czech Republic). Peatland degradation was locally caused by a combination of different factors (water level and chemistry) followed by vegetation succession, whose effect on fungi was evaluated using multivariate analyses. A total of 28 FINC species were detected using macrofungal surveying, whereas 25 were found using eDNA of Sphagnum and peat (Illumina sequencing of ITS2). These two methods agreed on 13 predominantly abundant FINC species, most of which grew in both well-preserved and degraded plots. However, their sensitivity to the studied degradation could not be assessed due to the low abundance of most species. Fungal communities found on the basis of both methods (macrofungal survey, eDNA) were affected by water chemistry, whereas a significant effect of vegetation and locally expansive plants was demonstrated only in fungal communities found using macrofungal survey. Optimisation (e.g. specific primers) for local conditions leading to a better FINC capture must be carried out if eDNA is to be used in the practice of evaluating management effects on peatlands.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/GA19-15031S" target="_blank" >GA19-15031S: Obnova biodiverzity narušených rašelinišť jako základ pro obnovu jejich budoucích ekosystémových funkcí a služeb</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mires and Peat

ISSN

1819-754X

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

2024

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

24

Strana od-do

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Kód UT WoS článku

001332999800001

EID výsledku v databázi Scopus

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