Counting sedimented Betula pollen grains by gas chromatography coupled with mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43909991" target="_blank" >RIV/60076658:12310/25:43909991 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10453-025-09855-z?utm_source=getftr&utm_medium=getftr&utm_campaign=getftr_pilot&getft_integrator=clarivate" target="_blank" >https://link.springer.com/article/10.1007/s10453-025-09855-z?utm_source=getftr&utm_medium=getftr&utm_campaign=getftr_pilot&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10453-025-09855-z" target="_blank" >10.1007/s10453-025-09855-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Counting sedimented Betula pollen grains by gas chromatography coupled with mass spectrometry

Popis výsledku v původním jazyce

Birch pollen grains (BPGs) are major aeroallergens in Europe, causing allergies in millions of people. Although background concentrations are provided by stationary pollen monitoring stations, they do not accurately reflect personal exposure. Knowing personal exposure makes it possible to establish a link between inhaled pollen grains and symptoms. Characterizing personal exposure to pollen using portable devices is challenging and requires time-consuming visual counting of pollen grains. We have developed a method for counting sedimented BPGs based on easy sampling using a handheld hoover and automated analysis by gas chromatography-mass spectrometry (GC-MS). This work is a feasibility study on the use of a lipid tracer (heptacosane) for the mass quantification of sedimented BPGs in outdoor and indoor environments. Before a lipid tracer could be used for the determination of BPGs, we ensured that the variability of total lipid mass was low (around 17%) for pollen samples from various geographical origins and for several pollen seasons. The limit of quantification of sedimented BPGs by GC-MS was estimated to be 100 mu g (equivalent to 16,000 BPGs), i.e. about 1.6 BPG cm-2 for a sampled surface of 1 m2. This method of assessing individual exposure was implemented during the birch pollen season indoors (between 70 and 225 sedimented BPGs per cm2) and outdoors, directly on the ground under a birch tree (over 6600 BPGs per cm2). This method of counting sedimented pollen grains is suitable for large sample series, and the data obtained could be used as an indicator of individual exposure to indoor air pollen in a large number of patients as part of epidemiological surveys.

Název v anglickém jazyce

Counting sedimented Betula pollen grains by gas chromatography coupled with mass spectrometry

Popis výsledku anglicky

Birch pollen grains (BPGs) are major aeroallergens in Europe, causing allergies in millions of people. Although background concentrations are provided by stationary pollen monitoring stations, they do not accurately reflect personal exposure. Knowing personal exposure makes it possible to establish a link between inhaled pollen grains and symptoms. Characterizing personal exposure to pollen using portable devices is challenging and requires time-consuming visual counting of pollen grains. We have developed a method for counting sedimented BPGs based on easy sampling using a handheld hoover and automated analysis by gas chromatography-mass spectrometry (GC-MS). This work is a feasibility study on the use of a lipid tracer (heptacosane) for the mass quantification of sedimented BPGs in outdoor and indoor environments. Before a lipid tracer could be used for the determination of BPGs, we ensured that the variability of total lipid mass was low (around 17%) for pollen samples from various geographical origins and for several pollen seasons. The limit of quantification of sedimented BPGs by GC-MS was estimated to be 100 mu g (equivalent to 16,000 BPGs), i.e. about 1.6 BPG cm-2 for a sampled surface of 1 m2. This method of assessing individual exposure was implemented during the birch pollen season indoors (between 70 and 225 sedimented BPGs per cm2) and outdoors, directly on the ground under a birch tree (over 6600 BPGs per cm2). This method of counting sedimented pollen grains is suitable for large sample series, and the data obtained could be used as an indicator of individual exposure to indoor air pollen in a large number of patients as part of epidemiological surveys.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

AEROBIOLOGIA

ISSN

0393-5965

e-ISSN

1573-3025

Svazek periodika

41

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

12

Strana od-do

333-344

Kód UT WoS článku

001467007700001

EID výsledku v databázi Scopus

2-s2.0-105002611574