Prevalence and diversity of Aphanomyces astaci in cambarid crayfish of Pennsylvania: where native and introduced hosts meet

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43910081" target="_blank" >RIV/60076658:12310/25:43910081 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10514331

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/parasitology/article/prevalence-and-diversity-of-aphanomyces-astaci-in-cambarid-crayfish-of-pennsylvania-where-native-and-introduced-hosts-meet/563DBCE3DBC5E72BC2A5C3A1E31D6678" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/prevalence-and-diversity-of-aphanomyces-astaci-in-cambarid-crayfish-of-pennsylvania-where-native-and-introduced-hosts-meet/563DBCE3DBC5E72BC2A5C3A1E31D6678</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182025000022" target="_blank" >10.1017/S0031182025000022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence and diversity of Aphanomyces astaci in cambarid crayfish of Pennsylvania: where native and introduced hosts meet

Popis výsledku v původním jazyce

The crayfish plague pathogen Aphanomyces astaci (Oomycota: Saprolegniales) is native to North America but expanded with its crayfish hosts to other regions. In most of its invaded range, A. astaci haplotypes are associated with specific American crayfish, probably due to introduction bottlenecks, but haplotype diversity is higher and clear host-specific associations are lacking in its native range. However, little is known about the infection rate and load of this pathogen in North America. We investigated the distribution, prevalence and genetic variation of A. astaci in Pennsylvania (eastern USA), where multiple native and introduced crayfish species (family Cambaridae) occur. We used A. astaci-specific quantitative PCR to screen 533 individuals representing 8 crayfish species (2 Cambarus and 6 Faxonius) from 49 sites. Faxonius limosus, an American species first introduced to Europe and carrier of A. astaci genotype group E, was of particular interest. We confirmed A. astaci infections in 76% of sites in all but 1 host taxon, with the pathogen infection rate and load comparable to established populations of North American crayfish studied in Europe and Japan. Despite the absence of highly infected hosts, we genotyped A. astaci from 14 sites. We only detected 2 mitochondrial haplotypes, but nuclear markers indicated the presence of at least 4 distinct pathogen genotypes, none documented from invaded areas in Europe or Asia. Genotype group E was not detected in F. limosus, possibly due to limited spatial distribution of the original strain. Our results highlight both benefits and limitations of combining multiple pathogen genotyping methods.

Název v anglickém jazyce

Prevalence and diversity of Aphanomyces astaci in cambarid crayfish of Pennsylvania: where native and introduced hosts meet

Popis výsledku anglicky

The crayfish plague pathogen Aphanomyces astaci (Oomycota: Saprolegniales) is native to North America but expanded with its crayfish hosts to other regions. In most of its invaded range, A. astaci haplotypes are associated with specific American crayfish, probably due to introduction bottlenecks, but haplotype diversity is higher and clear host-specific associations are lacking in its native range. However, little is known about the infection rate and load of this pathogen in North America. We investigated the distribution, prevalence and genetic variation of A. astaci in Pennsylvania (eastern USA), where multiple native and introduced crayfish species (family Cambaridae) occur. We used A. astaci-specific quantitative PCR to screen 533 individuals representing 8 crayfish species (2 Cambarus and 6 Faxonius) from 49 sites. Faxonius limosus, an American species first introduced to Europe and carrier of A. astaci genotype group E, was of particular interest. We confirmed A. astaci infections in 76% of sites in all but 1 host taxon, with the pathogen infection rate and load comparable to established populations of North American crayfish studied in Europe and Japan. Despite the absence of highly infected hosts, we genotyped A. astaci from 14 sites. We only detected 2 mitochondrial haplotypes, but nuclear markers indicated the presence of at least 4 distinct pathogen genotypes, none documented from invaded areas in Europe or Asia. Genotype group E was not detected in F. limosus, possibly due to limited spatial distribution of the original strain. Our results highlight both benefits and limitations of combining multiple pathogen genotyping methods.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

1469-8161

Svazek periodika

152

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

92-105

Kód UT WoS článku

001411598100001

EID výsledku v databázi Scopus

2-s2.0-85216177422