Determination of aliphatic aldehydes in maize and grass silages using on-fibre derivatisation with O-(2,3,4,5,6-pentafluoro)- benzylhydroxylamine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12410%2F09%3A00010694" target="_blank" >RIV/60076658:12410/09:00010694 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/09:00343022

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of aliphatic aldehydes in maize and grass silages using on-fibre derivatisation with O-(2,3,4,5,6-pentafluoro)- benzylhydroxylamine

Popis výsledku v původním jazyce

Aldehydes and ketones are volatile compounds of silages which probably affect feed preference, palatability and intake. A sensitive and fast method was developed to determine aliphatic aldehydes in maize and grass silages. The method is based on the solid phase micro-extraction and on-fibre derivatisation of aldehydes with O-(2,3,4,5,6-pentafluoro)benzylhydroxylamine hydrochloride (PFBHA). The derivatisation agent was adsorbed onto divinylbenzene/ poly(dimethylsiloxane) 65_m fibre and then exposed to the aldehydes in the headspace of the silage samples. Aldehydes selectively react with PFBHA forming volatile oximes, which were desorbed in the injection port of a gas chromatograph and analysed by mass spectrometry. Using this method, eight aldehydes were detected in maize and grass silages being ethanal, propanal, butanal, 2-methylpropanal, pentanal, 3-methylbutanal, hexanal and heptanal, at levels ranging fromtens to 200 mg/kg fresh matter. In two pilot laboratory experiments, contents

Název v anglickém jazyce

Determination of aliphatic aldehydes in maize and grass silages using on-fibre derivatisation with O-(2,3,4,5,6-pentafluoro)- benzylhydroxylamine

Popis výsledku anglicky

Aldehydes and ketones are volatile compounds of silages which probably affect feed preference, palatability and intake. A sensitive and fast method was developed to determine aliphatic aldehydes in maize and grass silages. The method is based on the solid phase micro-extraction and on-fibre derivatisation of aldehydes with O-(2,3,4,5,6-pentafluoro)benzylhydroxylamine hydrochloride (PFBHA). The derivatisation agent was adsorbed onto divinylbenzene/ poly(dimethylsiloxane) 65_m fibre and then exposed to the aldehydes in the headspace of the silage samples. Aldehydes selectively react with PFBHA forming volatile oximes, which were desorbed in the injection port of a gas chromatograph and analysed by mass spectrometry. Using this method, eight aldehydes were detected in maize and grass silages being ethanal, propanal, butanal, 2-methylpropanal, pentanal, 3-methylbutanal, hexanal and heptanal, at levels ranging fromtens to 200 mg/kg fresh matter. In two pilot laboratory experiments, contents

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Feed Science and Technology

ISSN

0377-8401

e-ISSN

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Svazek periodika

152

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000267479700016

EID výsledku v databázi Scopus

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