In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43872218" target="_blank" >RIV/60076658:12520/11:43872218 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.2478/v10102-011-0014-x" target="_blank" >http://dx.doi.org/10.2478/v10102-011-0014-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/v10102-011-0014-x" target="_blank" >10.2478/v10102-011-0014-x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol
Popis výsledku v původním jazyce
Sexually mature pigs are known to possess high concentrations of testicular steroids, which have been shown to change the activities of cytochrome P450 in vitro. The aim of the present study was to evaluate the regulation of CYP1A and CYP2E1 activity bythe steroids dihydrotestosterone (DHT), 3beta-androstenol, 17beta-estradiol and 17alfa-estradiol. Catalytic activities of 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) were used as markers of CYP1A activities, while p-nitrophenol hydroxylace (PNPH) was used as a marker of CYP2E1 activities. Of the tested steroids, only 17beta-estradiol and 17alfa-estradiol inhibited EROD and MROD activities. This inhibition was observed when a steroid concentration of 100 mýM was used, while lower concentration showed no inhibitory effect. PNPH activities were inhibited only by 100 mýM of 17beta-estradiol. The significance of those results in vivo is unknown because inhibition was only found when concentrations of oes
Název v anglickém jazyce
In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol
Popis výsledku anglicky
Sexually mature pigs are known to possess high concentrations of testicular steroids, which have been shown to change the activities of cytochrome P450 in vitro. The aim of the present study was to evaluate the regulation of CYP1A and CYP2E1 activity bythe steroids dihydrotestosterone (DHT), 3beta-androstenol, 17beta-estradiol and 17alfa-estradiol. Catalytic activities of 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) were used as markers of CYP1A activities, while p-nitrophenol hydroxylace (PNPH) was used as a marker of CYP2E1 activities. Of the tested steroids, only 17beta-estradiol and 17alfa-estradiol inhibited EROD and MROD activities. This inhibition was observed when a steroid concentration of 100 mýM was used, while lower concentration showed no inhibitory effect. PNPH activities were inhibited only by 100 mýM of 17beta-estradiol. The significance of those results in vivo is unknown because inhibition was only found when concentrations of oes
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Interdisciplinary Toxicology
ISSN
1337-6853
e-ISSN
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Svazek periodika
4
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
7
Strana od-do
78-84
Kód UT WoS článku
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EID výsledku v databázi Scopus
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