Potential role of the acrosome of sturgeon spermatozoa in the fertilization process

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43881323" target="_blank" >RIV/60076658:12520/11:43881323 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1439-0426.2010.01642.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0426.2010.01642.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0426.2010.01642.x" target="_blank" >10.1111/j.1439-0426.2010.01642.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Potential role of the acrosome of sturgeon spermatozoa in the fertilization process

Popis výsledku v původním jazyce

Sturgeon gametes are unique in that their spermatozoa possess acrosome whereas their eggs are perforated by numerous micropyles. Thus the function of acrosome is unclear. The acrosomal reaction of Acipenser ruthenus spermatozoa was induced by solution containing Ca2+ and high pH. The rate of affected spermatozoa was evaluated using Soybean Trypsin Inhibitor-Alexa conjugate fluorescent staining. Fertilization and hatching test proofed necessity of acrosome integrity for fertilization. Increasing percentage of spermatozoa with disrupted acrosome highly decreased percentage of hatching, in spite of electron microscopy verification that the spermatozoa bearing such disrupted acrosomes were able to reach the micropyle. In addition the significant positive correlation between the ability of spermatozoa to induce acrosomal reaction by activation solution and sperm fertilizing ability suggested that spermatozoa, which are able to activate the acrosomal reaction, are more successful in fertiliz

Název v anglickém jazyce

Potential role of the acrosome of sturgeon spermatozoa in the fertilization process

Popis výsledku anglicky

Sturgeon gametes are unique in that their spermatozoa possess acrosome whereas their eggs are perforated by numerous micropyles. Thus the function of acrosome is unclear. The acrosomal reaction of Acipenser ruthenus spermatozoa was induced by solution containing Ca2+ and high pH. The rate of affected spermatozoa was evaluated using Soybean Trypsin Inhibitor-Alexa conjugate fluorescent staining. Fertilization and hatching test proofed necessity of acrosome integrity for fertilization. Increasing percentage of spermatozoa with disrupted acrosome highly decreased percentage of hatching, in spite of electron microscopy verification that the spermatozoa bearing such disrupted acrosomes were able to reach the micropyle. In addition the significant positive correlation between the ability of spermatozoa to induce acrosomal reaction by activation solution and sperm fertilizing ability suggested that spermatozoa, which are able to activate the acrosomal reaction, are more successful in fertiliz

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

678-682

Kód UT WoS článku

000288864600087

EID výsledku v databázi Scopus

—