Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883408" target="_blank" >RIV/60076658:12520/12:43883408 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aquaculture.2012.05.006" target="_blank" >http://dx.doi.org/10.1016/j.aquaculture.2012.05.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2012.05.006" target="_blank" >10.1016/j.aquaculture.2012.05.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping

Popis výsledku v původním jazyce

We describe spermatozoa characteristics from sequential collections in sterlet, Acipenser ruthenus, following a single dose of carp pituitary extract (CPE). Sperm production and spermatozoa fertilizing ability, percent motility, and curvilinear velocity(VCL) were investigated in fresh and frozen/thawed sperm. Sperm was collected by two procedures: (A) stripping 3 times per 24 h at 3 h intervals on 3 consecutive days beginning 12 h after CPE treatment, and (B) stripping 3 times over 6 h beginning 36 h after CPE treatment. Spermatozoa motility and VCL were evaluated by video microscopy, and sperm production was measured as volume and concentration. Sperm samples were frozen by a conventional freezing procedure in a cryoprotective medium containing 10% methanol. Fertilization was conducted using a ratio of 105 spermatozoa/egg. Both sequential stripping procedures yielded larger volumes of viable spermatozoa than did a single collection. Sperm parameters such as density and volume varied

Název v anglickém jazyce

Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping

Popis výsledku anglicky

We describe spermatozoa characteristics from sequential collections in sterlet, Acipenser ruthenus, following a single dose of carp pituitary extract (CPE). Sperm production and spermatozoa fertilizing ability, percent motility, and curvilinear velocity(VCL) were investigated in fresh and frozen/thawed sperm. Sperm was collected by two procedures: (A) stripping 3 times per 24 h at 3 h intervals on 3 consecutive days beginning 12 h after CPE treatment, and (B) stripping 3 times over 6 h beginning 36 h after CPE treatment. Spermatozoa motility and VCL were evaluated by video microscopy, and sperm production was measured as volume and concentration. Sperm samples were frozen by a conventional freezing procedure in a cryoprotective medium containing 10% methanol. Fertilization was conducted using a ratio of 105 spermatozoa/egg. Both sequential stripping procedures yielded larger volumes of viable spermatozoa than did a single collection. Sperm parameters such as density and volume varied

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

356

Číslo periodika v rámci svazku

08

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

272-278

Kód UT WoS článku

000306171100036

EID výsledku v databázi Scopus

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