Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

Kód výsledku v IS VaVaI

RIV/60076658:12520/12:43883453 - isvavai.cz

Výsledek na webu

http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x

DOI - Digital Object Identifier

10.1111/j.1439-0426.2012.02021.x

Jazyk výsledku

angličtina

Název v původním jazyce

Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

Popis výsledku v původním jazyce

Feulgen image analysis densitometry (FIA) and image cytometry were used to study the relationship between the DNA content (pgDNA nucleus-1) and nuclear area (mu m2) in blood smears of evolutionary tetraploid (4n) sterlet (Acipenser ruthenus) and stellatesturgeon (A. stellatus); evolutionary octaploid (8n) Siberian sturgeon (A. baerii) and Russian sturgeon (A. gueldenstaedtii); hexaploid (6n) and decaploid (10n) fish found within A. baerii stock; and A. baerii and A. gueldenstaedtii exhibiting dodecaploidy (12n). Standards used for FIA were blood smears of chicken (Gallus gallus domesticus; 2.5 pgDNA nucleus-1) and diploid and induced triploid tench, Tinca tinca (2.04 and 3.1 pgDNA nucleus-1, respectively). All ploidy levels were first verified by means of flow cytometry. Species of the same ploidy level, however differing in their DNA content, exhibited a similar mean erythrocyte nuclear area, as could be demonstrated on A. ruthenus and A. stellatus (19.27 and 19.79 mu m2, respectivel

Název v anglickém jazyce

Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

Popis výsledku anglicky

Feulgen image analysis densitometry (FIA) and image cytometry were used to study the relationship between the DNA content (pgDNA nucleus-1) and nuclear area (mu m2) in blood smears of evolutionary tetraploid (4n) sterlet (Acipenser ruthenus) and stellatesturgeon (A. stellatus); evolutionary octaploid (8n) Siberian sturgeon (A. baerii) and Russian sturgeon (A. gueldenstaedtii); hexaploid (6n) and decaploid (10n) fish found within A. baerii stock; and A. baerii and A. gueldenstaedtii exhibiting dodecaploidy (12n). Standards used for FIA were blood smears of chicken (Gallus gallus domesticus; 2.5 pgDNA nucleus-1) and diploid and induced triploid tench, Tinca tinca (2.04 and 3.1 pgDNA nucleus-1, respectively). All ploidy levels were first verified by means of flow cytometry. Species of the same ploidy level, however differing in their DNA content, exhibited a similar mean erythrocyte nuclear area, as could be demonstrated on A. ruthenus and A. stellatus (19.27 and 19.79 mu m2, respectivel

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

704-708

Kód UT WoS článku

000308878000005

EID výsledku v databázi Scopus

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