Physiological functions of osmolality and calcium ions on the initiation of sperm motility and swimming performance in redside dace, Clinostomus elongatus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F13%3A43885193" target="_blank" >RIV/60076658:12520/13:43885193 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S109564331300127X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S109564331300127X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpa.2013.05.011" target="_blank" >10.1016/j.cbpa.2013.05.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physiological functions of osmolality and calcium ions on the initiation of sperm motility and swimming performance in redside dace, Clinostomus elongatus

Popis výsledku v původním jazyce

Reproductive potential of fish stocks is critically dependent on sperm performance in an aquatic environment. The aim of this study is to test hypotheses, which govern the initiation of sperm motility and swimming performance, through physiological functions of osmolality and Ca2+ ion, in a threatened species of freshwater fish, the redside dace, Clinostomus elongatus. Spermatozoa motility was activated in either ionic or non-ionic media spanning a range of osmolalities. The role of Ca2+ channels on induction of spermatozoa motility and velocity was experimentally investigated by diluting sperm in media that contain various Ca2+ channel blockers. Results show that initiation of spermatozoa motility is a hypo-osmolality dependent mechanism. Inhibitors for L-type Ca2+ channels partially prohibited initiation of spermatozoa motility, while velocity was significantly reduced in both L-type and T-type Ca2+ channel blockers. Examination using W-7, an inhibitor for Ca2+-dependent calmodulin,

Název v anglickém jazyce

Physiological functions of osmolality and calcium ions on the initiation of sperm motility and swimming performance in redside dace, Clinostomus elongatus

Popis výsledku anglicky

Reproductive potential of fish stocks is critically dependent on sperm performance in an aquatic environment. The aim of this study is to test hypotheses, which govern the initiation of sperm motility and swimming performance, through physiological functions of osmolality and Ca2+ ion, in a threatened species of freshwater fish, the redside dace, Clinostomus elongatus. Spermatozoa motility was activated in either ionic or non-ionic media spanning a range of osmolalities. The role of Ca2+ channels on induction of spermatozoa motility and velocity was experimentally investigated by diluting sperm in media that contain various Ca2+ channel blockers. Results show that initiation of spermatozoa motility is a hypo-osmolality dependent mechanism. Inhibitors for L-type Ca2+ channels partially prohibited initiation of spermatozoa motility, while velocity was significantly reduced in both L-type and T-type Ca2+ channel blockers. Examination using W-7, an inhibitor for Ca2+-dependent calmodulin,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology

ISSN

1095-6433

e-ISSN

—

Svazek periodika

166

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

147-157

Kód UT WoS článku

000322693200018

EID výsledku v databázi Scopus

—