In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F13%3A43885225" target="_blank" >RIV/60076658:12520/13:43885225 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S1532045613000525" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1532045613000525</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpc.2013.05.002" target="_blank" >10.1016/j.cbpc.2013.05.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

Popis výsledku v původním jazyce

Among endocrine disruptors, the xenoestrogen bisphenol A (BPA) deserves particular attention due to widespread human exposure. Besides hormonal effects, BPA has been suspected to be responsible for adverse effect on reproductive ability of various species. In the present study the effect of BPA on the quality parameters, oxidative stress, the DNA integrity and intracellular ATP content of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were exposed to concentrationsof BPA possibly occurring in nature (0.5, 1.75, 25, 5 and 10 mu g/L) for 2 h. Results revealed that BPA significantly decreased spermatozoa motility and velocity of spermatozoa at concentration of EPA 2.5-10 mu g/L. Significant positive correlation (r =0.713, P { 0.05) was found between percent motile spermatozoa and ATP content. Oxidative stress was observed at concentrations 1.75-10 mu g/L, as reflected by significantly higher levels of protein and lipid oxidation and superoxide dism

Název v anglickém jazyce

In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

Popis výsledku anglicky

Among endocrine disruptors, the xenoestrogen bisphenol A (BPA) deserves particular attention due to widespread human exposure. Besides hormonal effects, BPA has been suspected to be responsible for adverse effect on reproductive ability of various species. In the present study the effect of BPA on the quality parameters, oxidative stress, the DNA integrity and intracellular ATP content of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were exposed to concentrationsof BPA possibly occurring in nature (0.5, 1.75, 25, 5 and 10 mu g/L) for 2 h. Results revealed that BPA significantly decreased spermatozoa motility and velocity of spermatozoa at concentration of EPA 2.5-10 mu g/L. Significant positive correlation (r =0.713, P { 0.05) was found between percent motile spermatozoa and ATP content. Oxidative stress was observed at concentrations 1.75-10 mu g/L, as reflected by significantly higher levels of protein and lipid oxidation and superoxide dism

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology C - Toxicology and Pharmacology

ISSN

1532-0456

e-ISSN

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Svazek periodika

158

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

64-71

Kód UT WoS článku

000321936900002

EID výsledku v databázi Scopus

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