Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886830" target="_blank" >RIV/60076658:12520/14:43886830 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/jai.12429/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jai.12429/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jai.12429" target="_blank" >10.1111/jai.12429</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca)

Popis výsledku v původním jazyce

Embryonic and larval development of the tench, Tinca tinca, is described, along with the origin and migration routes of germ cell lineage. Primordial germ cells (PGCs) potentially transmit genetic information to the next generation, and represent a powerful tool for creating a germ-line chimera within fish species. PGCs were identified by injecting synthesized mRNA, combining green fluorescent protein and the zebrafish nos1 3UTR, under the blastodisc of embryos at the 1-4 cell stage. Developmental stages were divided into five periods defined by morphological features: cleavage (40min-5h post-fertilization (hpf), blastula (3.6-12hpf), gastrula (12-20hpf), segmentation (20-65hpf), and hatching (65-188hpf). The migration pathways of fluorescent PGCs weredetected from 100% epiboly (18hpf) to the end of the hatching period (184hpf) in 69.3% of injected embryos, which migrated to the site of future gonads. Each hatching larva possessed 3-10 labeled PGCs with 95.1% of these cells localized

Název v anglickém jazyce

Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca)

Popis výsledku anglicky

Embryonic and larval development of the tench, Tinca tinca, is described, along with the origin and migration routes of germ cell lineage. Primordial germ cells (PGCs) potentially transmit genetic information to the next generation, and represent a powerful tool for creating a germ-line chimera within fish species. PGCs were identified by injecting synthesized mRNA, combining green fluorescent protein and the zebrafish nos1 3UTR, under the blastodisc of embryos at the 1-4 cell stage. Developmental stages were divided into five periods defined by morphological features: cleavage (40min-5h post-fertilization (hpf), blastula (3.6-12hpf), gastrula (12-20hpf), segmentation (20-65hpf), and hatching (65-188hpf). The migration pathways of fluorescent PGCs weredetected from 100% epiboly (18hpf) to the end of the hatching period (184hpf) in 69.3% of injected embryos, which migrated to the site of future gonads. Each hatching larva possessed 3-10 labeled PGCs with 95.1% of these cells localized

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

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Svazek periodika

30

Číslo periodika v rámci svazku

Supplement 1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

29-39

Kód UT WoS článku

000337527600007

EID výsledku v databázi Scopus

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