The Effect of Reactive Oxygen Species on Motility Parameters, DNA Integrity, Tyrosine Phosphorylation and Phosphatase Activity of Common Carp (Cyprinus carpio L.) Spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888471" target="_blank" >RIV/60076658:12520/15:43888471 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/mrd.22442/abstract;jsessionid=1CDC2A472DF296DAD4A24E74D6C47297.f02t01" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/mrd.22442/abstract;jsessionid=1CDC2A472DF296DAD4A24E74D6C47297.f02t01</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrd.22442" target="_blank" >10.1002/mrd.22442</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Reactive Oxygen Species on Motility Parameters, DNA Integrity, Tyrosine Phosphorylation and Phosphatase Activity of Common Carp (Cyprinus carpio L.) Spermatozoa

Popis výsledku v původním jazyce

The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed todifferent concentrations of xanthine and xanthine oxidase (X-XO) either in the presence or absence of antioxidants for 15 and 60min. A dose- and time-dependent reduction in spermatozoa motility and velocity was observed. Comet assays showed a dramatic increase in DNA fragmentation after 15min. Changes in tyrosine phosphorylation of spermatozoa proteins were observed by Western blotting with anti-phosphotyrosine antibodies, and proteins of interest were identified by mass spectrometry. After a 60min exposure to X-XO, O-linked N-acetylglucosamine transferase, isoform 4 was phosphorylated and septin-8-A was dephosphorylated. Acid phosphatase activity also decreased in a dose-dependent manner after a 60min exposure to oxidative stress. The

Název v anglickém jazyce

The Effect of Reactive Oxygen Species on Motility Parameters, DNA Integrity, Tyrosine Phosphorylation and Phosphatase Activity of Common Carp (Cyprinus carpio L.) Spermatozoa

Popis výsledku anglicky

The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed todifferent concentrations of xanthine and xanthine oxidase (X-XO) either in the presence or absence of antioxidants for 15 and 60min. A dose- and time-dependent reduction in spermatozoa motility and velocity was observed. Comet assays showed a dramatic increase in DNA fragmentation after 15min. Changes in tyrosine phosphorylation of spermatozoa proteins were observed by Western blotting with anti-phosphotyrosine antibodies, and proteins of interest were identified by mass spectrometry. After a 60min exposure to X-XO, O-linked N-acetylglucosamine transferase, isoform 4 was phosphorylated and septin-8-A was dephosphorylated. Acid phosphatase activity also decreased in a dose-dependent manner after a 60min exposure to oxidative stress. The

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

48-57

Kód UT WoS článku

000348656700006

EID výsledku v databázi Scopus

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