Novel Technique for Visualizing Primordial Germ Cells in Sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888642" target="_blank" >RIV/60076658:12520/15:43888642 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biolreprod.org/content/93/4/96" target="_blank" >http://www.biolreprod.org/content/93/4/96</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1095/biolreprod.115.128314" target="_blank" >10.1095/biolreprod.115.128314</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel Technique for Visualizing Primordial Germ Cells in Sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso)

Popis výsledku v původním jazyce

Primordial germ cells (PGCs) are the origin of all germ cells in developing embryos. In the sturgeon embryo, PGCs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. Current methods for studying sturgeon PGCsrequire either killing the fish or using costly and time-consuming histological procedures. Here, we demonstrate that visualization of sterlet (Acipenser ruthenus) PGCs in vivo is feasible by simply labeling the vegetal hemisphere with fluorescein isothiocyanate (FITC)-dextran. We injected FITC-dextrans, with molecular weights varying between 10 000 and 2 000 000, into the vegetal pole of 1- to 4-cell stage embryos. At the neurula to tail-bud developmental stages, FITC-positive PGC-like cells appeared ventrally around the developing tail bud in the experimental group that received a high-molecular-weight FITC-dextran. The highest average number of FITC-positive PGC-like cells was observed in embryos injected with FITC-dextran having a m

Název v anglickém jazyce

Novel Technique for Visualizing Primordial Germ Cells in Sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso)

Popis výsledku anglicky

Primordial germ cells (PGCs) are the origin of all germ cells in developing embryos. In the sturgeon embryo, PGCs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. Current methods for studying sturgeon PGCsrequire either killing the fish or using costly and time-consuming histological procedures. Here, we demonstrate that visualization of sterlet (Acipenser ruthenus) PGCs in vivo is feasible by simply labeling the vegetal hemisphere with fluorescein isothiocyanate (FITC)-dextran. We injected FITC-dextrans, with molecular weights varying between 10 000 and 2 000 000, into the vegetal pole of 1- to 4-cell stage embryos. At the neurula to tail-bud developmental stages, FITC-positive PGC-like cells appeared ventrally around the developing tail bud in the experimental group that received a high-molecular-weight FITC-dextran. The highest average number of FITC-positive PGC-like cells was observed in embryos injected with FITC-dextran having a m

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Svazek periodika

93

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000364587700002

EID výsledku v databázi Scopus

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