Quantification of adenosine triphosphate, adenosine diphosphate, and creatine phosphate in sterlet Acipenser ruthenus spermatozoa during maturation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888847" target="_blank" >RIV/60076658:12520/15:43888847 - isvavai.cz</a>

Výsledek na webu

<a href="https://dl.sciencesocieties.org/publications/jas/abstracts/93/11/5214" target="_blank" >https://dl.sciencesocieties.org/publications/jas/abstracts/93/11/5214</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2527/jas2015-9144" target="_blank" >10.2527/jas2015-9144</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of adenosine triphosphate, adenosine diphosphate, and creatine phosphate in sterlet Acipenser ruthenus spermatozoa during maturation

Popis výsledku v původním jazyce

Sturgeon spermatozoa maturation during their passage through the kidney is a prerequisite for initiation of motility. Samples of sterlet (Acipenser ruthenus) testicular sperm (TS) were matured in vitro by incubation in seminal fluid (SF) or in SF supplemented with carbonyl cyanide m-chlorophenyl hydrazone (CCCP; a respiration uncoupling agent). Sperm was diluted in activation medium (AM) containing 10 mM Tris-HCl buffer (pH 8.5) and 0.25% Pluronic, and spermatozoon motility was assessed. Samples were taken and fixed in 3 M perchloric acid at 3 points in the incubation process. Quantification of ATP, ADP, and creatine phosphate (CrP) was conducted using liquid chromatography/high-resolution mass spectrometry. We observed a significant decrease in CrP during artificial maturation of TS in SF. In contrast, ATP and ADP were not significantly affected. Addition of CCCP to SF halted maturation and led to significantly lower CrP whereas ADP significantly increased and ATP was unaffected. Dilu

Název v anglickém jazyce

Quantification of adenosine triphosphate, adenosine diphosphate, and creatine phosphate in sterlet Acipenser ruthenus spermatozoa during maturation

Popis výsledku anglicky

Sturgeon spermatozoa maturation during their passage through the kidney is a prerequisite for initiation of motility. Samples of sterlet (Acipenser ruthenus) testicular sperm (TS) were matured in vitro by incubation in seminal fluid (SF) or in SF supplemented with carbonyl cyanide m-chlorophenyl hydrazone (CCCP; a respiration uncoupling agent). Sperm was diluted in activation medium (AM) containing 10 mM Tris-HCl buffer (pH 8.5) and 0.25% Pluronic, and spermatozoon motility was assessed. Samples were taken and fixed in 3 M perchloric acid at 3 points in the incubation process. Quantification of ATP, ADP, and creatine phosphate (CrP) was conducted using liquid chromatography/high-resolution mass spectrometry. We observed a significant decrease in CrP during artificial maturation of TS in SF. In contrast, ATP and ADP were not significantly affected. Addition of CCCP to SF halted maturation and led to significantly lower CrP whereas ADP significantly increased and ATP was unaffected. Dilu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal Science

ISSN

0021-8812

e-ISSN

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Svazek periodika

93

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

5214-5221

Kód UT WoS článku

000366326300011

EID výsledku v databázi Scopus

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