Technical note: Production of tetraploid sturgeons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888852" target="_blank" >RIV/60076658:12520/15:43888852 - isvavai.cz</a>

Výsledek na webu

<a href="https://dl.sciencesocieties.org/publications/jas/abstracts/93/8/3759" target="_blank" >https://dl.sciencesocieties.org/publications/jas/abstracts/93/8/3759</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2527/jas.2015-9094" target="_blank" >10.2527/jas.2015-9094</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Technical note: Production of tetraploid sturgeons

Popis výsledku v původním jazyce

Studies and practical application of androgenesis and gynogenesis in sturgeon are significantly hindered by strong influence of ploidy restoration treatment on survivability of progeny; therefore, developed method of production of tetraploid broodstock and, consequently, use of their diploid gametes might help to avoid ploidy restoration treatment. In the present study, for the first time was developed a protocol for tetraploidy induction in 2 model sturgeon species, sterlet (Acipenser ruthenus) and Siberian sturgeon (Acipenser baerii). A high efficiency of treatment was achieved by optimization of heat shock using a temperature of 37 degrees C for 2 min timed between the end of female pronuclei formation and the beginning of pronuclei migration, thatis, 0.8 to 1.0 tau(0) (duration of 1 mitotic cycle during the period of synchronous cleavage division). Fertilized eggs developed in tetraploid larvae, up to 31 (89.6% in control) and 34% (70.9% in control) in sterlet and Siberian sturgeo

Název v anglickém jazyce

Technical note: Production of tetraploid sturgeons

Popis výsledku anglicky

Studies and practical application of androgenesis and gynogenesis in sturgeon are significantly hindered by strong influence of ploidy restoration treatment on survivability of progeny; therefore, developed method of production of tetraploid broodstock and, consequently, use of their diploid gametes might help to avoid ploidy restoration treatment. In the present study, for the first time was developed a protocol for tetraploidy induction in 2 model sturgeon species, sterlet (Acipenser ruthenus) and Siberian sturgeon (Acipenser baerii). A high efficiency of treatment was achieved by optimization of heat shock using a temperature of 37 degrees C for 2 min timed between the end of female pronuclei formation and the beginning of pronuclei migration, thatis, 0.8 to 1.0 tau(0) (duration of 1 mitotic cycle during the period of synchronous cleavage division). Fertilized eggs developed in tetraploid larvae, up to 31 (89.6% in control) and 34% (70.9% in control) in sterlet and Siberian sturgeo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal Science

ISSN

0021-8812

e-ISSN

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Svazek periodika

93

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

3759-3764

Kód UT WoS článku

000365171300002

EID výsledku v databázi Scopus

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