Identification of the light-independent phosphoserine pathway as an additional source of serine in the cyanobacterium Synechocystis sp PCC 6803

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888887" target="_blank" >RIV/60076658:12520/15:43888887 - isvavai.cz</a>

Výsledek na webu

<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000055" target="_blank" >http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/mic.0.000055" target="_blank" >10.1099/mic.0.000055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of the light-independent phosphoserine pathway as an additional source of serine in the cyanobacterium Synechocystis sp PCC 6803

Popis výsledku v původním jazyce

L-Serine is one of the proteinogenic amino acids and participates in several essential processes in all organisms. In plants, the light-dependent photorespiratory and the light-independent phosphoserine pathways contribute to serine biosynthesis. In cyanobacteria, the light-dependent photorespiratory pathway for serine synthesis is well characterized, but the phosphoserine pathway has not been identified. Here, we investigated three candidate genes for enzymes of the phosphoserine pathway in Synechocystis sp. PCC 6803. Only the gene for the D-3-phosphoglycerate dehydrogenase is correctly annotated in the genome database, whereas the 3-phosphoserine transaminase and 3-phosphoserine phosphatase (PSP) proteins are incorrectly annotated and were identifiedhere. All enzymes were obtained as recombinant proteins and showed the activities necessary to catalyse the three-step phosphoserine pathway. The genes coding for the phosphoserine pathway were found in most cyanobacterial genomes listed

Název v anglickém jazyce

Identification of the light-independent phosphoserine pathway as an additional source of serine in the cyanobacterium Synechocystis sp PCC 6803

Popis výsledku anglicky

L-Serine is one of the proteinogenic amino acids and participates in several essential processes in all organisms. In plants, the light-dependent photorespiratory and the light-independent phosphoserine pathways contribute to serine biosynthesis. In cyanobacteria, the light-dependent photorespiratory pathway for serine synthesis is well characterized, but the phosphoserine pathway has not been identified. Here, we investigated three candidate genes for enzymes of the phosphoserine pathway in Synechocystis sp. PCC 6803. Only the gene for the D-3-phosphoglycerate dehydrogenase is correctly annotated in the genome database, whereas the 3-phosphoserine transaminase and 3-phosphoserine phosphatase (PSP) proteins are incorrectly annotated and were identifiedhere. All enzymes were obtained as recombinant proteins and showed the activities necessary to catalyse the three-step phosphoserine pathway. The genes coding for the phosphoserine pathway were found in most cyanobacterial genomes listed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbiology-SGM

ISSN

1350-0872

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1050-1060

Kód UT WoS článku

000358186900012

EID výsledku v databázi Scopus

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