Epidermal growth factor enhances the developmental competence of yak (Bos grunniens) preimplantation embryos by modulating the expression of survivin and HSP70
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43890330" target="_blank" >RIV/60076658:12520/15:43890330 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S1871141315300287" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1871141315300287</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.livsci.2015.11.002" target="_blank" >10.1016/j.livsci.2015.11.002</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Epidermal growth factor enhances the developmental competence of yak (Bos grunniens) preimplantation embryos by modulating the expression of survivin and HSP70
Popis výsledku v původním jazyce
Culture conditions can affect cleavage, zygotic genome activation, embryonic development, and blastocyst quality. Epidermal growth factor (EGF) has important autocrine and/or paracrine effects during early embryonic development. However, few studies of the effect of EGF on yak embryos have been reported. The aim of this study was to determine the effects of exogenous EGF on yak embryonic development in vitro, as well as the correlation between HSP70 and survivin expression patterns, blastocyst quality and EGF concentration. The in vitro matured cumulus-oocyte complexes (COCs) were fertilized with frozen-thawed semen, and the zygotes were incubated in culture media supplemented with different concentrations of EGF. During in vitro culture, the embryos were analyzed for development to the 4-cell, 8-cell, 16-cell, and blastocyst stages. The variation in total blastocyst cell numbers and their allocation to the inner cell mass (ICM) and trophectoderm (TE) lineages were determined by differ
Název v anglickém jazyce
Epidermal growth factor enhances the developmental competence of yak (Bos grunniens) preimplantation embryos by modulating the expression of survivin and HSP70
Popis výsledku anglicky
Culture conditions can affect cleavage, zygotic genome activation, embryonic development, and blastocyst quality. Epidermal growth factor (EGF) has important autocrine and/or paracrine effects during early embryonic development. However, few studies of the effect of EGF on yak embryos have been reported. The aim of this study was to determine the effects of exogenous EGF on yak embryonic development in vitro, as well as the correlation between HSP70 and survivin expression patterns, blastocyst quality and EGF concentration. The in vitro matured cumulus-oocyte complexes (COCs) were fertilized with frozen-thawed semen, and the zygotes were incubated in culture media supplemented with different concentrations of EGF. During in vitro culture, the embryos were analyzed for development to the 4-cell, 8-cell, 16-cell, and blastocyst stages. The variation in total blastocyst cell numbers and their allocation to the inner cell mass (ICM) and trophectoderm (TE) lineages were determined by differ
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Livestock Science
ISSN
1871-1413
e-ISSN
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Svazek periodika
182
Číslo periodika v rámci svazku
Neuveden
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
118-124
Kód UT WoS článku
000366776500018
EID výsledku v databázi Scopus
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